Quantum biology: An update and perspective
Understanding the rules of life is one of the most important scientific endeavours and has
revolutionised both biology and biotechnology. Remarkable advances in observation …
revolutionised both biology and biotechnology. Remarkable advances in observation …
Cell-based assays: fuelling drug discovery
It has been estimated that over a billion dollars in resources can be consumed to obtain
clinical approval, and only a few new chemical entities are approved by the US Food and …
clinical approval, and only a few new chemical entities are approved by the US Food and …
Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%
J Goedhart, D Von Stetten, M Noirclerc-Savoye… - Nature …, 2012 - nature.com
Cyan variants of green fluorescent protein are widely used as donors in Förster resonance
energy transfer experiments. The popular, but modestly bright, Enhanced Cyan Fluorescent …
energy transfer experiments. The popular, but modestly bright, Enhanced Cyan Fluorescent …
[HTML][HTML] Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent protein voltage probe
Monitoring neuronal electrical activity using fluorescent protein-based voltage sensors has
been limited by small response magnitudes and slow kinetics of existing probes. Here we …
been limited by small response magnitudes and slow kinetics of existing probes. Here we …
Nr4a1 and Nr4a3 reporter mice are differentially sensitive to T cell receptor signal strength and duration
E Jennings, TAE Elliot, N Thawait, S Kanabar… - Cell reports, 2020 - cell.com
Nr4a receptors are activated by T cell receptor (TCR) signaling and play key roles in T cell
differentiation. Which TCR signaling pathways regulate Nr4a receptors and their sensitivities …
differentiation. Which TCR signaling pathways regulate Nr4a receptors and their sensitivities …
GFP fluorescence peak fraction analysis based nanothermometer for the assessment of exothermal mitochondria activity in live cells
Nanothermometry methods with intracellular sensitivities have the potential to make
important contributions to fundamental cell biology and medical fields, as temperature is a …
important contributions to fundamental cell biology and medical fields, as temperature is a …
Flow cytometry of fluorescent proteins
Fluorescent proteins are now a critical tool in all areas of biomedical research. In this article,
we review the techniques required to use fluorescent proteins for flow cytometry …
we review the techniques required to use fluorescent proteins for flow cytometry …
Stimuli-responsive smart gels realized via modular protein design
Smart gels have a variety of applications, including tissue engineering and controlled drug
delivery. Here we present a modular, bottom-up approach that permits the creation of protein …
delivery. Here we present a modular, bottom-up approach that permits the creation of protein …
Environmental and nuclear influences on microalgal chloroplast gene expression
Microalgal chloroplasts, such as those of the model organism Chlamydomonas reinhardtii,
are emerging as a new platform to produce recombinant proteins, including industrial …
are emerging as a new platform to produce recombinant proteins, including industrial …
Multiplex T cell stimulation assay utilizing a T cell activation reporter-based detection system
SE Mann, Z Zhou, LG Landry, AM Anderson… - Frontiers in …, 2020 - frontiersin.org
Recent advancements in single cell sequencing technologies allow for identification of
numerous immune-receptors expressed by T cells such as tumor-specific and autoimmune T …
numerous immune-receptors expressed by T cells such as tumor-specific and autoimmune T …