A guide to light-sheet fluorescence microscopy for multiscale imaging

RM Power, J Huisken - Nature methods, 2017 - nature.com
The impact of light-sheet fluorescence microscopy (LSFM) is visible in fields as diverse as
developmental and cell biology, anatomical science, biophysics and neuroscience …

Light-sheet microscopy: a tutorial

OE Olarte, J Andilla, EJ Gualda… - Advances in Optics and …, 2018 - opg.optica.org
This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from
an optics perspective. As such, emphasis is placed on the advantages that LS microscope …

The 2015 super-resolution microscopy roadmap

SW Hell, SJ Sahl, M Bates, X Zhuang… - Journal of Physics D …, 2015 - iopscience.iop.org
Far-field optical microscopy using focused light is an important tool in a number of scientific
disciplines including chemical,(bio) physical and biomedical research, particularly with …

A versatile tiling light sheet microscope for imaging of cleared tissues

Y Chen, X Li, D Zhang, C Wang, R Feng, X Li, Y Wen… - Cell Reports, 2020 - cell.com
We present a tiling light sheet microscope compatible with all tissue clearing methods for
rapid multicolor 3D imaging of cleared tissues with micron-scale (4× 4× 10 μm 3) to …

How to define and optimize axial resolution in light-sheet microscopy: a simulation-based approach

E Remacha, L Friedrich, J Vermot… - Biomedical optics …, 2019 - opg.optica.org
“How thick is your light sheet?” is a question that has been asked frequently after talks
showing impressive renderings of 3D data acquired by a light-sheet microscope. This …

Frontiers in artificial intelligence‐directed light‐sheet microscopy for uncovering biological phenomena and multiorgan imaging

E Zhu, YR Li, S Margolis, J Wang, K Wang, Y Zhang… - View, 2024 - Wiley Online Library
Light‐sheet fluorescence microscopy (LSFM) introduces fast scanning of biological
phenomena with deep photon penetration and minimal phototoxicity. This advancement …

Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy

Q Fu, BL Martin, DQ Matus, L Gao - Nature communications, 2016 - nature.com
Despite the progress made in selective plane illumination microscopy, high-resolution 3D
live imaging of multicellular specimens remains challenging. Tiling light-sheet selective …

Cancellation of Bessel beam side lobes for high-contrast light sheet microscopy

G Di Domenico, G Ruocco, C Colosi, E DelRe… - Scientific reports, 2018 - nature.com
An ideal illumination for light sheet fluorescence microscopy entails both a localized and a
propagation invariant optical field. Bessel beams and Airy beams satisfy these conditions …

Successful 3D imaging of cleared biological samples with light sheet fluorescence microscopy

E Delage, T Guilbert, F Yates - Journal of Cell Biology, 2023 - rupress.org
In parallel with the development of tissue-clearing methods, over the last decade, light sheet
fluorescence microscopy has contributed to major advances in various fields, such as cell …

Lattice light sheet microscopy using tiling lattice light sheets

L Gao, WC Tang, YC Tsai, BC Chen - Optics Express, 2019 - opg.optica.org
We present a novel method used to implement tiling lattice light sheets (LLS) in lattice light
sheet microscopy (LLSM) on regular LLS microscopes without changing the LLS …