A guide to light-sheet fluorescence microscopy for multiscale imaging
RM Power, J Huisken - Nature methods, 2017 - nature.com
The impact of light-sheet fluorescence microscopy (LSFM) is visible in fields as diverse as
developmental and cell biology, anatomical science, biophysics and neuroscience …
developmental and cell biology, anatomical science, biophysics and neuroscience …
Light-sheet microscopy: a tutorial
This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from
an optics perspective. As such, emphasis is placed on the advantages that LS microscope …
an optics perspective. As such, emphasis is placed on the advantages that LS microscope …
The 2015 super-resolution microscopy roadmap
Far-field optical microscopy using focused light is an important tool in a number of scientific
disciplines including chemical,(bio) physical and biomedical research, particularly with …
disciplines including chemical,(bio) physical and biomedical research, particularly with …
A versatile tiling light sheet microscope for imaging of cleared tissues
Y Chen, X Li, D Zhang, C Wang, R Feng, X Li, Y Wen… - Cell Reports, 2020 - cell.com
We present a tiling light sheet microscope compatible with all tissue clearing methods for
rapid multicolor 3D imaging of cleared tissues with micron-scale (4× 4× 10 μm 3) to …
rapid multicolor 3D imaging of cleared tissues with micron-scale (4× 4× 10 μm 3) to …
How to define and optimize axial resolution in light-sheet microscopy: a simulation-based approach
E Remacha, L Friedrich, J Vermot… - Biomedical optics …, 2019 - opg.optica.org
“How thick is your light sheet?” is a question that has been asked frequently after talks
showing impressive renderings of 3D data acquired by a light-sheet microscope. This …
showing impressive renderings of 3D data acquired by a light-sheet microscope. This …
Frontiers in artificial intelligence‐directed light‐sheet microscopy for uncovering biological phenomena and multiorgan imaging
Light‐sheet fluorescence microscopy (LSFM) introduces fast scanning of biological
phenomena with deep photon penetration and minimal phototoxicity. This advancement …
phenomena with deep photon penetration and minimal phototoxicity. This advancement …
Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy
Despite the progress made in selective plane illumination microscopy, high-resolution 3D
live imaging of multicellular specimens remains challenging. Tiling light-sheet selective …
live imaging of multicellular specimens remains challenging. Tiling light-sheet selective …
Cancellation of Bessel beam side lobes for high-contrast light sheet microscopy
An ideal illumination for light sheet fluorescence microscopy entails both a localized and a
propagation invariant optical field. Bessel beams and Airy beams satisfy these conditions …
propagation invariant optical field. Bessel beams and Airy beams satisfy these conditions …
Successful 3D imaging of cleared biological samples with light sheet fluorescence microscopy
E Delage, T Guilbert, F Yates - Journal of Cell Biology, 2023 - rupress.org
In parallel with the development of tissue-clearing methods, over the last decade, light sheet
fluorescence microscopy has contributed to major advances in various fields, such as cell …
fluorescence microscopy has contributed to major advances in various fields, such as cell …
Lattice light sheet microscopy using tiling lattice light sheets
We present a novel method used to implement tiling lattice light sheets (LLS) in lattice light
sheet microscopy (LLSM) on regular LLS microscopes without changing the LLS …
sheet microscopy (LLSM) on regular LLS microscopes without changing the LLS …