Voltage imaging with genetically encoded indicators
Highlights•Recent advances in genetically encoded voltage indicators (GEVIs) have brought
neural voltage imaging in vivo within reach.•Recently introduced tools such as electrically …
neural voltage imaging in vivo within reach.•Recently introduced tools such as electrically …
One gene, many neuropsychiatric disorders: lessons from Mendelian diseases
Recent human genetic studies have consistently shown that mutations in the same gene or
same genomic region can increase the risk of a broad range of complex neuropsychiatric …
same genomic region can increase the risk of a broad range of complex neuropsychiatric …
Photoactivated voltage imaging in tissue with an archaerhodopsin-derived reporter
Photoactivated genetically encoded voltage indicators (GEVIs) have the potential to enable
optically sectioned voltage imaging at the intersection of a photoactivation beam and an …
optically sectioned voltage imaging at the intersection of a photoactivation beam and an …
Photophysics-informed two-photon voltage imaging using FRET-opsin voltage indicators
Microbial rhodopsin–derived genetically encoded voltage indicators (GEVIs) are powerful
tools for map** bioelectrical dynamics in cell culture and in live animals. Förster …
tools for map** bioelectrical dynamics in cell culture and in live animals. Förster …
Two-photon lifetime imaging of voltage indicating proteins as a probe of absolute membrane voltage
Genetically encoded voltage indicators (GEVIs) can report cellular electrophysiology with
high resolution in space and time. Two-photon (2P) fluorescence has been explored as a …
high resolution in space and time. Two-photon (2P) fluorescence has been explored as a …
All-optical electrophysiology for high-throughput functional characterization of a human iPSC-derived motor neuron model of ALS
Human induced pluripotent stem cell (iPSC)-derived neurons are an attractive substrate for
modeling disease, yet the heterogeneity of these cultures presents a challenge for functional …
modeling disease, yet the heterogeneity of these cultures presents a challenge for functional …
Multiplexed temporally focused light sha** for high-resolution multi-cell targeting
Optical wavefront sha** is a powerful technique to control the distribution of light in the
focus of a microscope. This ability, combined with optogenetics, holds great promise for …
focus of a microscope. This ability, combined with optogenetics, holds great promise for …
Microbial rhodopsins
I Gushchin, V Gordeliy - Membrane Protein Complexes: Structure and …, 2018 - Springer
Microbial rhodopsins (MRs) are a large family of photoactive membrane proteins, found in
microorganisms belonging to all kingdoms of life, with new members being constantly …
microorganisms belonging to all kingdoms of life, with new members being constantly …
Synthetic and genetically encoded fluorescent neural activity indicators
Highlights•Fluorescence microscopy is an excellent tool to monitor neuronal
activity.•Multiple synthetic and genetically encoded indicators are available.•The utility of …
activity.•Multiple synthetic and genetically encoded indicators are available.•The utility of …
Extracting structural and functional features of widely distributed biological circuits with single cell resolution via tissue clearing and delivery vectors
Highlights•Tissue clearing and viral vectors for resolved 3D imaging of unsevered
circuits.•Size-adjustable tissue-hydrogels for sample stabilization and selective …
circuits.•Size-adjustable tissue-hydrogels for sample stabilization and selective …