The power of any genetic model organism is derived, in part, from the ease with which gene
expression can be manipulated. The short generation time and invariant developmental …

Mutagenesis drives natural selection. In the lab, mutations allow gene function to be
deciphered. C. elegans is highly amendable to functional genetics because of its short …

To the Editor: The ability to add or delete genes to the genome of genetic model organisms
is essential. Previously, we had developed methods based on the Mos1 transposon1 to …

Genome editing based on CRISPR (clustered regularly interspaced short palindromic
repeats)-associated nuclease (Cas9) has been successfully applied in dozens of diverse …

As in other organisms, CRISPR/Cas9 methods provide a powerful approach for genome
editing in the nematode Caenorhabditis elegans. Oligonucleotides are excellent repair …

We have generated a recombinant Mos1 transposon that can insert up to 45-kb transgenes
into the Caenorhabditis elegans genome. The minimal Mos1 transposon (miniMos) is 550 …

Expanding the Genetic Code of an Animal | Journal of the American Chemical Society ACS ACS
Publications C&EN CAS Find my institution Log In Journal of the American Chemical Society …

Efficient and reproducible transgenesis facilitates and accelerates research using genetic
model organisms. Here, we describe a mo dular s afe-harbor t ransgene i nsertion (MosTI) …

Here we describe a toolkit for the production of fluorescently tagged proteins in the C.
elegans germline and early embryo using Mos1-mediated single copy insertion (MosSCI) …

Use of the CRISPR/Cas9 RNA-guided endonuclease complex has recently enabled the
generation of double-strand breaks virtually anywhere in the C. elegans genome. Here, we …