Analyzing high-dimensional cytometry data using FlowSOM
The dimensionality of cytometry data has strongly increased in the last decade, and in many
situations the traditional manual downstream analysis becomes insufficient. The field is …
situations the traditional manual downstream analysis becomes insufficient. The field is …
Flow cytometry: the next revolution
Unmasking the subtleties of the immune system requires both a comprehensive knowledge
base and the ability to interrogate that system with intimate sensitivity. That task, to a …
base and the ability to interrogate that system with intimate sensitivity. That task, to a …
[HTML][HTML] Dynamic CD8+ T cell responses to cancer immunotherapy in human regional lymph nodes are disrupted in metastatic lymph nodes
CD8+ T cell responses are critical for anti-tumor immunity. While extensively profiled in the
tumor microenvironment, recent studies in mice identified responses in lymph nodes (LNs) …
tumor microenvironment, recent studies in mice identified responses in lymph nodes (LNs) …
[HTML][HTML] A blood atlas of COVID-19 defines hallmarks of disease severity and specificity
DJ Ahern, Z Ai, M Ainsworth, C Allan, A Allcock… - Cell, 2022 - cell.com
Treatment of severe COVID-19 is currently limited by clinical heterogeneity and incomplete
description of specific immune biomarkers. We present here a comprehensive multi-omic …
description of specific immune biomarkers. We present here a comprehensive multi-omic …
Multi-omic profiling reveals widespread dysregulation of innate immunity and hematopoiesis in COVID-19
Our understanding of protective versus pathological immune responses to SARS-CoV-2, the
virus that causes coronavirus disease 2019 (COVID-19), is limited by inadequate profiling of …
virus that causes coronavirus disease 2019 (COVID-19), is limited by inadequate profiling of …
Integrated immune dynamics define correlates of COVID-19 severity and antibody responses
SARS-CoV-2 causes a spectrum of COVID-19 disease, the immunological basis of which
remains ill defined. We analyzed 85 SARS-CoV-2-infected individuals at acute and/or …
remains ill defined. We analyzed 85 SARS-CoV-2-infected individuals at acute and/or …
Immune signatures predict development of autoimmune toxicity in patients with cancer treated with immune checkpoint inhibitors
NG Nunez, F Berner, E Friebel, S Unger, N Wyss… - Med, 2023 - cell.com
Summary Background Immune checkpoint inhibitors (ICIs) are among the most promising
treatment options for melanoma and non-small cell lung cancer (NSCLC). While ICIs can …
treatment options for melanoma and non-small cell lung cancer (NSCLC). While ICIs can …
Guidelines for the use of flow cytometry and cell sorting in immunological studies
The third edition of Flow Cytometry Guidelines provides the key aspects to consider when
performing flow cytometry experiments and includes comprehensive sections describing …
performing flow cytometry experiments and includes comprehensive sections describing …
Integration, exploration, and analysis of high‐dimensional single‐cell cytometry data using Spectre
As the size and complexity of high‐dimensional (HD) cytometry data continue to expand,
comprehensive, scalable, and methodical computational analysis approaches are essential …
comprehensive, scalable, and methodical computational analysis approaches are essential …
An updated guide for the perplexed: cytometry in the high-dimensional era
High-dimensional cytometry experiments measuring 20–50 cellular markers have become
routine in many laboratories. The increased complexity of these datasets requires added …
routine in many laboratories. The increased complexity of these datasets requires added …