The CRISPR tool kit for genome editing and beyond
M Adli - Nature communications, 2018 - nature.com
CRISPR is becoming an indispensable tool in biological research. Once known as the
bacterial immune system against invading viruses, the programmable capacity of the Cas9 …
bacterial immune system against invading viruses, the programmable capacity of the Cas9 …
CRISPR-based technologies for the manipulation of eukaryotic genomes
The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of
advances in the life sciences that have grown from the ability to edit genomes within living …
advances in the life sciences that have grown from the ability to edit genomes within living …
Map** the energetic and allosteric landscapes of protein binding domains
Allosteric communication between distant sites in proteins is central to biological regulation
but still poorly characterized, limiting understanding, engineering and drug development …
but still poorly characterized, limiting understanding, engineering and drug development …
New CRISPR–Cas systems from uncultivated microbes
CRISPR–Cas systems provide microbes with adaptive immunity by employing short DNA
sequences, termed spacers, that guide Cas proteins to cleave foreign DNA,. Class 2 …
sequences, termed spacers, that guide Cas proteins to cleave foreign DNA,. Class 2 …
CasX enzymes comprise a distinct family of RNA-guided genome editors
The RNA-guided CRISPR-associated (Cas) proteins Cas9 and Cas12a provide adaptive
immunity against invading nucleic acids, and function as powerful tools for genome editing …
immunity against invading nucleic acids, and function as powerful tools for genome editing …
Cornerstones of CRISPR–Cas in drug discovery and therapy
The recent development of CRISPR–Cas systems as easily accessible and programmable
tools for genome editing and regulation is spurring a revolution in biology. Paired with the …
tools for genome editing and regulation is spurring a revolution in biology. Paired with the …
Controlling and enhancing CRISPR systems
Many bacterial and archaeal organisms use clustered regularly interspaced short
palindromic repeats–CRISPR associated (CRISPR–Cas) systems to defend themselves …
palindromic repeats–CRISPR associated (CRISPR–Cas) systems to defend themselves …
Engineered LwaCas13a with enhanced collateral activity for nucleic acid detection
Clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated
protein 13 (Cas13) has been rapidly developed for nucleic-acid-based diagnostics by using …
protein 13 (Cas13) has been rapidly developed for nucleic-acid-based diagnostics by using …
Increasing Cas9-mediated homology-directed repair efficiency through covalent tethering of DNA repair template
The CRISPR-Cas9 system is a powerful genome-editing tool in which a guide RNA targets
Cas9 to a site in the genome, where the Cas9 nuclease then induces a double-stranded …
Cas9 to a site in the genome, where the Cas9 nuclease then induces a double-stranded …
Engineering self-deliverable ribonucleoproteins for genome editing in the brain
The delivery of CRISPR ribonucleoproteins (RNPs) for genome editing in vitro and in vivo
has important advantages over other delivery methods, including reduced off-target and …
has important advantages over other delivery methods, including reduced off-target and …