Optical imaging is an indispensable tool in clinical diagnostics and fundamental biomedical
research. Autofluorescence-free optical imaging, which eliminates real-time optical …

Bioluminescence is the fascinating natural phenomenon by which living creatures produce
light. Bioluminescence occurs when the oxidation of a small-molecule luciferin is catalysed …

Regulatable CAR platforms could circumvent toxicities associated with CAR-T therapy, but
existing systems have shortcomings including leakiness and attenuated activity. Here, we …

Genetically encoded voltage indicators are emerging tools for monitoring voltage dynamics
with cell-type specificity. However, current indicators enable a narrow range of applications …

Adoptive cell therapy (ACT) has proven to be highly effective in treating blood cancers, but
traditional approaches to ACT are poorly effective in treating solid tumors observed …

Fluorescent RNAs, aptamers that bind and activate small fluorogenic dyes, have provided a
particularly attractive approach to visualizing RNAs in live cells. However, the simultaneous …

Afterglow optical agents, which emit light long after cessation of excitation, hold promise for
ultrasensitive in vivo imaging because they eliminate tissue autofluorescence. However …

Cellular signaling networks are the foundation which determines the fate and function of
cells as they respond to various cues and stimuli. The discovery of fluorescent proteins over …

Bioluminescence is a natural light source based on luciferase catalysis of its substrate
luciferin. We performed directed evolution on firefly luciferase using a red-shifted and highly …

Comprehensively resolving neuronal identities in whole-brain images is a major challenge.
We achieve this in C. elegans by engineering a multicolor transgene called NeuroPAL (a …