Homing gene drives can transfer rapidly between Anopheles gambiae strains with minimal carryover of flanking sequences

P Pescod, G Bevivino, A Anthousi, J Shepherd… - Nature …, 2024 - nature.com
CRISPR-Cas9 homing gene drives are designed to induce a targeted double-stranded DNA
break at a wild type allele ('recipient'), which, when repaired by the host cell, is converted to …

Transgene removal using an in cis programmed homing endonuclease via single-strand annealing in the mosquito Aedes aegypti

K Chae, B Contreras, JS Romanowski… - Communications …, 2024 - nature.com
While gene drive strategies have been proposed to aid in the control of mosquito-borne
diseases, additional genome engineering technologies may be required to establish a …

DNA Damage Response Mechanisms in Model Systems

JR LaRocque, M McVey - Genes, 2023 - mdpi.com
Cells are constantly assaulted by endogenous and exogenous sources of DNA damage that
threaten genome stability. To maintain genome integrity, organisms have evolved a myriad …

The epistatic relationship of Drosophila melanogaster CtIP and Rif1 in homology-directed repair of DNA double-strand breaks

MS Thomas, GS Pillai, MA Butler… - G3: Genes …, 2024 - academic.oup.com
Double-strand breaks (DSBs) are genotoxic DNA lesions that pose significant threats to
genomic stability, necessitating precise and efficient repair mechanisms to prevent cell …

Homing-based gene drives can introgress rapidly into local genetic backgrounds with minimal chromosomal conversion in Anopheles gambiae

T Nolan, P Pescod, G Bevivino, A Anthousi, J Shepherd… - 2023 - researchsquare.com
CRISPR-Cas9 gene drive control strategies use a homing selfish genetic element which
induces a double-stranded break at the target site and is copied into the opposing …

[PDF][PDF] Proteomic insights into spliceosome components involved in antisense transcription at DNA breaks

P Đurić - 2023 - edoc.ub.uni-muenchen.de
Small interfering RNAs (siRNAs) are expressed directly at DNA breaks in multiple eukaryotic
species. This opens questions on their expression mechanism in Drosophila, where they …