An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes

NR Mohamad, NHC Marzuki, NA Buang… - Biotechnology & …, 2015 - Taylor & Francis
The current demands of sustainable green methodologies have increased the use of
enzymatic technology in industrial processes. Employment of enzyme as biocatalysts offers …

Biomaterials via peptide assembly: Design, characterization, and application in tissue engineering

VP Gray, CD Amelung, IJ Duti, EG Laudermilch… - Acta biomaterialia, 2022 - Elsevier
A core challenge in biomaterials, with both fundamental significance and technological
relevance, concerns the rational design of bioactive microenvironments. Designed properly …

Self-assembly of peptide nanocapsules by a solvent concentration gradient

H Li, X Qian, H Mohanram, X Han, H Qi, G Zou… - Nature …, 2024 - nature.com
Biological systems can create materials with intricate structures and specialized functions. In
comparison, precise control of structures in human-made materials has been challenging …

Energy landscapes and functions of supramolecular systems

F Tantakitti, J Boekhoven, X Wang, RV Kazantsev… - Nature materials, 2016 - nature.com
By means of two supramolecular systems—peptide amphiphiles engaged in hydrogen-
bonded β-sheets, and chromophore amphiphiles driven to assemble by π-orbital overlaps …

Using circular dichroism collected as a function of temperature to determine the thermodynamics of protein unfolding and binding interactions

NJ Greenfield - Nature protocols, 2006 - nature.com
Circular dichroism (CD) is an excellent spectroscopic technique for following the unfolding
and folding of proteins as a function of temperature. One of its principal applications is to …

Protein helical structure determination using CD spectroscopy for solutions with strong background absorbance from 190 to 230 nm

Y Wei, AA Thyparambil, RA Latour - … et Biophysica Acta (BBA)-Proteins and …, 2014 - Elsevier
Conventional empirical methods for the quantification of the helical content of proteins in
solution using circular dichroism (CD) primarily rely on spectral data acquired between …

Non-conventional fluorescent biogenic and synthetic polymers without aromatic rings

R Ye, Y Liu, H Zhang, H Su, Y Zhang, L Xu, R Hu… - Polymer …, 2017 - pubs.rsc.org
Non-conventional fluorescent materials without aromatic structures have attracted much
research attention in recent years. However, the working mechanism responsible for their …

Physicochemical and antioxidant properties of industrial hemp seed protein isolate treated by high-intensity ultrasound

G Karabulut, H Feng, O Yemiş - Plant Foods for Human Nutrition, 2022 - Springer
Ultrasound is one of the non-thermal, green, and novel technologies used to functionalize
plant proteins. We recently determined the optimum conditions of high-intensity ultrasound …

[KNYGA][B] Basics of enzyme immobilization

A Dwevedi, A Dwevedi - 2016 - Springer
Immobilization of enzymes is not a new concept but one that has been around for over 100
years. However, wide applications for immobilized enzymes came only in the past four …

Engineered single-domain antibodies with high protease resistance and thermal stability

G Hussack, T Hirama, W Ding, R MacKenzie, J Tanha - PloS one, 2011 - journals.plos.org
The extreme pH and protease-rich environment of the upper gastrointestinal tract is a major
obstacle facing orally-administered protein therapeutics, including antibodies. Through …