Message in a bubble: shuttling small RNAs and proteins between cells and interacting organisms using extracellular vesicles

Q Cai, B He, S Wang, S Fletcher, D Niu… - Annual review of …, 2021 - annualreviews.org
Communication between plant cells and interacting microorganisms requires the secretion
and uptake of functional molecules to and from the extracellular environment and is …

Lab-to-field transition of RNA spray applications–how far are we?

AP Rank, A Koch - Frontiers in Plant Science, 2021 - frontiersin.org
The drastic loss of biodiversity has alarmed the public and raised sociopolitical demand for
chemical pesticide-free plant production, which is now treated by governments worldwide as …

Nanoparticle stereochemistry-dependent endocytic processing improves in vivo mRNA delivery

MZC Hatit, CN Dobrowolski, MP Lokugamage… - Nature Chemistry, 2023 - nature.com
Stereochemistry can alter small-molecule pharmacokinetics, safety and efficacy. However, it
is unclear whether the stereochemistry of a single compound within a multicomponent …

RNA interference past and future applications in plants

S Koeppe, L Kawchuk, M Kalischuk - International Journal of Molecular …, 2023 - mdpi.com
Antisense RNA was observed to elicit plant disease resistance and post-translational gene
silencing (PTGS). The universal mechanism of RNA interference (RNAi) was shown to be …

dsRNA uptake in plant pests and pathogens: insights into RNAi-based insect and fungal control technology

N Wytinck, CL Manchur, VH Li, S Whyard, MF Belmonte - Plants, 2020 - mdpi.com
Efforts to develop more environmentally friendly alternatives to traditional broad-spectrum
pesticides in agriculture have recently turned to RNA interference (RNAi) technology. With …

Improving RNA-based crop protection through nanotechnology and insights from cross-kingdom RNA trafficking

A Chen, L Halilovic, JH Shay, A Koch, N Mitter… - Current opinion in plant …, 2023 - Elsevier
Spray-induced gene silencing (SIGS) is a powerful and eco-friendly method for crop
protection. Based off the discovery of RNA uptake ability in many fungal pathogens, the …

[HTML][HTML] Requirements for fungal uptake of dsRNA and gene silencing in RNAi-based crop protection strategies

E Šečić, KH Kogel - Current Opinion in Biotechnology, 2021 - Elsevier
Highlights•RNAi-based plant protection against fungal pathogens relies on uptake and
processing of dsRNA molecules by fungal hyphae.•Silencing is mediated by dsRNAs …

Exogenous double‐stranded RNA inhibits the infection physiology of rust fungi to reduce symptoms in planta

RM Degnan, AR McTaggart, LS Shuey… - Molecular Plant …, 2023 - Wiley Online Library
Rust fungi (Pucciniales) are a diverse group of plant pathogens in natural and agricultural
systems. They pose ongoing threats to the diversity of native flora and cause annual crop …

Double‐stranded RNA targeting fungal ergosterol biosynthesis pathway controls Botrytis cinerea and postharvest grey mould

D Duanis‐Assaf, O Galsurker… - Plant biotechnology …, 2022 - Wiley Online Library
Pathogenic fungi cause major postharvest losses. During storage and ripening, fruit
becomes highly susceptible to fungi that cause postharvest disease. Fungicides are effective …

Extracellular RNA: mechanisms of secretion and potential functions

ML Borniego, RW Innes - Journal of experimental botany, 2023 - academic.oup.com
Extracellular RNA (exRNA) has long been considered as cellular waste that plants can
degrade and utilize to recycle nutrients. However, recent findings highlight the need to …