1H-Detected Biomolecular NMR under Fast Magic-Angle Spinning
T Le Marchand, T Schubeis, M Bonaccorsi… - Chemical …, 2022 - ACS Publications
Since the first pioneering studies on small deuterated peptides dating more than 20 years
ago, 1H detection has evolved into the most efficient approach for investigation of …
ago, 1H detection has evolved into the most efficient approach for investigation of …
Solid-state NMR investigations of extracellular matrixes and cell walls of algae, bacteria, fungi, and plants
Extracellular matrixes (ECMs), such as the cell walls and biofilms, are important for
supporting cell integrity and function and regulating intercellular communication. These …
supporting cell integrity and function and regulating intercellular communication. These …
Structure of fully protonated proteins by proton-detected magic-angle spinning NMR
Protein structure determination by proton-detected magic-angle spinning (MAS) NMR has
focused on highly deuterated samples, in which only a small number of protons are …
focused on highly deuterated samples, in which only a small number of protons are …
Atomic-resolution chemical characterization of (2x)72-kDa tryptophan synthase via four- and five-dimensional 1H-detected solid-state NMR
NMR chemical shifts provide detailed information on the chemical properties of molecules,
thereby complementing structural data from techniques like X-ray crystallography and …
thereby complementing structural data from techniques like X-ray crystallography and …
Deuteration for high-resolution detection of protons in protein magic angle spinning (MAS) solid-state NMR
B Reif - Chemical Reviews, 2021 - ACS Publications
Proton detection developed in the last 20 years as the method of choice to study
biomolecules in the solid state. In perdeuterated proteins, proton dipolar interactions are …
biomolecules in the solid state. In perdeuterated proteins, proton dipolar interactions are …
Spinning proteins, the faster, the better?
A Böckmann, M Ernst, BH Meier - Journal of Magnetic Resonance, 2015 - Elsevier
Magic-angle spinning (MAS) is a technique that is a prerequisite for high-resolution solid-
state NMR spectroscopy of proteins and other biomolecules. Recently, the 100 kHz limit for …
state NMR spectroscopy of proteins and other biomolecules. Recently, the 100 kHz limit for …
NMR spectroscopic assignment of backbone and side‐chain protons in fully protonated proteins: microcrystals, sedimented assemblies, and amyloid fibrils
We demonstrate sensitive detection of alpha protons of fully protonated proteins by solid‐
state NMR spectroscopy with 100–111 kHz magic‐angle spinning (MAS). The excellent …
state NMR spectroscopy with 100–111 kHz magic‐angle spinning (MAS). The excellent …
High-resolution proton-detected NMR of proteins at very fast MAS
When combined with high-frequency (currently∼ 60 kHz) magic-angle spinning (MAS),
proton detection boosts sensitivity and increases coherence lifetimes, resulting in narrow 1 …
proton detection boosts sensitivity and increases coherence lifetimes, resulting in narrow 1 …
Structure and gating behavior of the human integral membrane protein VDAC1 in a lipid bilayer
The voltage-dependent anion channel (VDAC), the most abundant protein in the outer
mitochondrial membrane, is responsible for the transport of all ions and metabolites into and …
mitochondrial membrane, is responsible for the transport of all ions and metabolites into and …
Structure and Mechanism of the Influenza A M218–60 Dimer of Dimers
We report a magic angle spinning (MAS) NMR structure of the drug-resistant S31N mutation
of M218–60 from Influenza A. The protein was dispersed in diphytanoyl-sn-glycero-3 …
of M218–60 from Influenza A. The protein was dispersed in diphytanoyl-sn-glycero-3 …