Type IV secretion systems: advances in structure, function, and activation

TRD Costa, L Harb, P Khara, L Zeng, B Hu… - Molecular …, 2021 - Wiley Online Library
Bacterial type IV secretion systems (T4SSs) are a functionally diverse translocation
superfamily. They consist mainly of two large subfamilies:(i) conjugation systems that …

From conjugation to T4S systems in Gram‐negative bacteria: a mechanistic biology perspective

G Waksman - EMBO reports, 2019 - embopress.org
Conjugation is the process by which bacteria exchange genetic materials in a unidirectional
manner from a donor cell to a recipient cell. The discovery of conjugation signalled the dawn …

Cryo-EM structure of a type IV secretion system

K Macé, AK Vadakkepat, A Redzej, N Lukoyanova… - Nature, 2022 - nature.com
Bacterial conjugation is the fundamental process of unidirectional transfer of DNAs, often
plasmid DNAs, from a donor cell to a recipient cell. It is the primary means by which …

Structure of a type IV secretion system core complex encoded by multi-drug resistance F plasmids

X Liu, P Khara, ML Baker, PJ Christie, B Hu - Nature communications, 2022 - nature.com
Bacterial type IV secretion systems (T4SSs) are largely responsible for the proliferation of
multi-drug resistance. We solved the structure of the outer-membrane core complex …

The rich tapestry of bacterial protein translocation systems

PJ Christie - The protein journal, 2019 - Springer
The translocation of proteins across membranes is a fundamental cellular function. Bacteria
have evolved a striking array of pathways for delivering proteins into or across cytoplasmic …

In Situ Molecular Architecture of the Helicobacter pylori Cag Type IV Secretion System

B Hu, P Khara, L Song, AS Lin, AE Frick-Cheng… - MBio, 2019 - Am Soc Microbiol
Helicobacter pylori colonizes about half of humans worldwide, and its presence in the
gastric mucosa is associated with an increased risk of gastric adenocarcinoma, gastric …

Biological and structural diversity of type IV secretion systems

YG Li, B Hu, PJ Christie - Microbiology spectrum, 2019 - Am Soc Microbiol
The bacterial type IV secretion systems (T4SSs) are a functionally diverse superfamily of
secretion systems found in many species of bacteria. Collectively, the T4SSs translocate …

In Situ Visualization of the pKM101-Encoded Type IV Secretion System Reveals a Highly Symmetric ATPase Energy Center

P Khara, L Song, PJ Christie, B Hu - MBio, 2021 - Am Soc Microbiol
Bacterial conjugation systems are members of the type IV secretion system (T4SS)
superfamily. T4SSs can be classified as “minimized” or “expanded” based on whether they …

Chimeric systems composed of swapped Tra subunits between distantly-related F plasmids reveal striking plasticity among type IV secretion machines

K Kishida, YG Li, N Ogawa-Kishida, P Khara… - PLoS …, 2024 - journals.plos.org
Bacterial type IV secretion systems (T4SSs) are a versatile family of macromolecular
translocators, collectively able to recruit diverse DNA and protein substrates and deliver …

Cryo-EM structure of a conjugative type IV secretion system suggests a molecular switch regulating pilus biogenesis

K Macé, G Waksman - The EMBO Journal, 2024 - embopress.org
Conjugative type IV secretion systems (T4SS) mediate bacterial conjugation, a process that
enables the unidirectional exchange of genetic materials between a donor and a recipient …