[HTML][HTML] A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming

DM Lyra-Leite, Ó Gutiérrez-Gutiérrez, M Wang, Y Zhou… - STAR protocols, 2022 - Elsevier
The methods for the culture and cardiomyocyte differentiation of human embryonic stem
cells, and later human induced pluripotent stem cells (hiPSC), have moved from a complex …

Next generation of heart regenerative therapies: progress and promise of cardiac tissue engineering

MF Tenreiro, AF Louro, PM Alves… - NPJ Regenerative …, 2021 - nature.com
The adult heart is a vital and highly specialized organ of the human body, with limited
capability of self-repair and regeneration in case of injury or disease. Engineering …

Metabolic communication by SGLT2 inhibition

AM Billing, YC Kim, S Gullaksen, B Schrage, J Raabe… - Circulation, 2024 - ahajournals.org
BACKGROUND: SGLT2 (sodium-glucose cotransporter 2) inhibitors (SGLT2i) can protect
the kidneys and heart, but the underlying mechanism remains poorly understood …

Biomanufacturing of organ-specific tissues with high cellular density and embedded vascular channels

MA Skylar-Scott, SGM Uzel, LL Nam, JH Ahrens… - Science …, 2019 - science.org
Engineering organ-specific tissues for therapeutic applications is a grand challenge,
requiring the fabrication and maintenance of densely cellular constructs composed of~ 108 …

Self-assembling human heart organoids for the modeling of cardiac development and congenital heart disease

YR Lewis-Israeli, AH Wasserman, MA Gabalski… - Nature …, 2021 - nature.com
Congenital heart defects constitute the most common human birth defect, however
understanding of how these disorders originate is limited by our ability to model the human …

Metabolic maturation media improve physiological function of human iPSC-derived cardiomyocytes

DAM Feyen, WL McKeithan, AAN Bruyneel, S Spiering… - Cell reports, 2020 - cell.com
Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) have enormous potential
for the study of human cardiac disorders. However, their physiological immaturity severely …

SARS-CoV-2 infects and induces cytotoxic effects in human cardiomyocytes

D Bojkova, JUG Wagner, M Shumliakivska… - Cardiovascular …, 2020 - academic.oup.com
Aims Coronavirus disease 2019 is caused by severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2) and has emerged as a global pandemic. SARS-CoV-2 …

SARS-CoV-2 infects human engineered heart tissues and models COVID-19 myocarditis

AL Bailey, O Dmytrenko, L Greenberg… - Basic to Translational …, 2021 - jacc.org
There is ongoing debate as to whether cardiac complications of coronavirus disease-2019
(COVID-19) result from myocardial viral infection or are secondary to systemic inflammation …

Efficient and reproducible generation of human iPSC-derived cardiomyocytes and cardiac organoids in stirred suspension systems

M Prondzynski, P Berkson, MA Trembley… - Nature …, 2024 - nature.com
Human iPSC-derived cardiomyocytes (hiPSC-CMs) have proven invaluable for cardiac
disease modeling and regeneration. Challenges with quality, inter-batch consistency …

Human engineered heart tissue patches remuscularize the injured heart in a dose-dependent manner

E Querdel, M Reinsch, L Castro, D Köse, A Bähr… - Circulation, 2021 - ahajournals.org
Background: Human engineered heart tissue (EHT) transplantation represents a potential
regenerative strategy for patients with heart failure and has been successful in preclinical …