Integrase and integration: biochemical activities of HIV-1 integrase
Integration of retroviral DNA is an obligatory step of retrovirus replication because proviral
DNA is the template for productive infection. Integrase, a retroviral enzyme, catalyses …
DNA is the template for productive infection. Integrase, a retroviral enzyme, catalyses …
Slee** beauty transposition: biology and applications for molecular therapy
Transposable elements can be considered as natural, nonviral gene-delivery vehicles and
are valuable and widely used tools for germ-line transgenesis and insertional mutagenesis …
are valuable and widely used tools for germ-line transgenesis and insertional mutagenesis …
Common physical properties of DNA affecting target site selection of slee** beauty and other Tc1/mariner transposable elements
Slee** Beauty (SB) is the most active Tc1/mariner-type transposable element in
vertebrates, and is therefore a valuable vector for transposon mutagenesis in vertebrate …
vertebrates, and is therefore a valuable vector for transposon mutagenesis in vertebrate …
Development of hyperactive slee** beauty transposon vectors by mutational analysis
Abstract The Slee** Beauty (SB) transposable element is a promising vector for
transgenesis in vertebrates and is being developed as a novel, nonviral system for gene …
transgenesis in vertebrates and is being developed as a novel, nonviral system for gene …
[HTML][HTML] Sequence specificity of viral end DNA binding by HIV-1 integrase reveals critical regions for protein–DNA interaction
D Esposito, R Craigie - The EMBO journal, 1998 - embopress.org
HIV-1 integrase specifically recognizes and cleaves viral end DNA during the initial step of
retroviral integration. The protein and DNA determinants of the specificity of viral end DNA …
retroviral integration. The protein and DNA determinants of the specificity of viral end DNA …
Gene therapy for severe combined immunodeficiency: are we there yet?
M Cavazzana-Calvo, A Fischer - The Journal of clinical …, 2007 - Am Soc Clin Investig
Inherited and acquired diseases of the hematopoietic system can be cured by allogeneic
hematopoietic stem cell transplantation. This treatment strategy is highly successful when an …
hematopoietic stem cell transplantation. This treatment strategy is highly successful when an …
Integrating DNA: transposases and retroviral integrases
L Haren, B Ton-Hoang… - Annual Reviews in …, 1999 - annualreviews.org
▪ Abstract Transposable elements appear quite disparate in their organization and in the
types of genetic rearrangements they promote. In spite of this diversity, retroviruses and …
types of genetic rearrangements they promote. In spite of this diversity, retroviruses and …
Tn5/IS50 target recognition
IY Goryshin, JA Miller, YV Kil… - Proceedings of the …, 1998 - National Acad Sciences
This communication reports an analysis of Tn 5/IS 50 target site selection by using an
extensive collection of Tn 5 and IS 50 insertions in two relatively small regions of DNA (less …
extensive collection of Tn 5 and IS 50 insertions in two relatively small regions of DNA (less …
Engineered Slee** Beauty transposase redirects transposon integration away from genes
C Miskey, L Kesselring, I Querques… - Nucleic acids …, 2022 - academic.oup.com
Abstract The Slee** Beauty (SB) transposon system is a popular tool for genome
engineering, but random integration into the genome carries a certain genotoxic risk in …
engineering, but random integration into the genome carries a certain genotoxic risk in …
[HTML][HTML] Tn10 transposition via a DNA hairpin intermediate
AK Kennedy, A Guhathakurta, N Kleckner, DB Haniford - Cell, 1998 - cell.com
We present evidence that excision of the nonreplicative transposon Tn10 involves three
distinct chemical steps, first-strand nicking, hairpin formation, and hairpin resolution. This …
distinct chemical steps, first-strand nicking, hairpin formation, and hairpin resolution. This …