Microfluidic chip electrophoresis for biochemical analysis

X Ou, P Chen, X Huang, S Li… - Journal of separation …, 2020 - Wiley Online Library
Microfluidic chip electrophoresis has been widely employed for separation of various
biochemical species owing to its advantages of low sample consumption, low cost, fast …

Micro total analysis systems: fundamental advances and biological applications

CT Culbertson, TG Mickleburgh… - Analytical …, 2014 - ACS Publications
It has been more than 20 years since the first micro total analysis systems (μTAS) papers
were published. Initial reports of these devices, which are also commonly referred to as …

Present state of microchip electrophoresis: state of the art and routine applications

ER Castro, A Manz - Journal of Chromatography A, 2015 - Elsevier
Microchip electrophoresis (MCE) was one of the earliest applications of the micro-total
analysis system (μ-TAS) concept, whose aim is to reduce analysis time and reagent and …

The development of microfluidic-based western blotting: Technical advances and future perspectives

CT Desire, RD Arrua, XL Strudwick, Z Kopecki… - … of Chromatography A, 2023 - Elsevier
Over the past two decades significant technical advancement in the field of western blotting
has been made possible through the utilization of microfluidic technologies. In this review …

Detection of isoforms differing by a single charge unit in individual cells

AM Tentori, KA Yamauchi… - Angewandte Chemie …, 2016 - Wiley Online Library
To measure protein isoforms in individual mammalian cells, we report single‐cell resolution
isoelectric focusing (scIEF) and high‐selectivity immunoprobing. Microfluidic design and …

Polymer sieving matrices in microanalytical electrophoresis

M Chung, D Kim, AE Herr - Analyst, 2014 - pubs.rsc.org
Microfluidic design has advanced existing protein separation capabilities and supported
novel assays. Key metrics for successful protein separations include fast, robust, and …

Reducing Cathodic Drift during Isoelectric Focusing Using Microscale Immobilized pH Gradient Gels

G Lomeli, AE Herr - Analytical Chemistry, 2024 - ACS Publications
Microfluidic analytical tools play an important role in miniaturizing targeted proteomic assays
for improved detection sensitivity, throughput, and automation. Microfluidic isoelectric …

[HTML][HTML] Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications

JD Urbano-Gámez, F Perdigones, JM Quero - Micromachines, 2021 - mdpi.com
In this paper, a prototype of a semi-automatic lab-on-PCB for agarose gel preparation and
electrophoresis is developed. The dimensions of the device are 38× 34 mm 2 and it includes …

Capacitively coupled contactless conductivity detection for microfluidic capillary isoelectric focusing

MK Chau, NG Arega, NAN Tran, J Song, S Lee… - Analytica Chimica …, 2020 - Elsevier
We report capacitively coupled contactless conductivity detection (C4D) of proteins
separated by microfluidic capillary isoelectric focusing (μCIEF). To elucidate the evolution of …

Recent advances in microscale western blotting

BJ Sanders, DC Kim, RC Dunn - Analytical Methods, 2016 - pubs.rsc.org
Western blotting is a ubiquitous tool used extensively in the clinical and research settings to
identify proteins and characterize their levels. It has rapidly become a mainstay in research …