Abiotic stress in crop production

R Kopecká, M Kameniarová, M Černý… - International Journal of …, 2023‏ - mdpi.com
The vast majority of agricultural land undergoes abiotic stress that can significantly reduce
agricultural yields. Understanding the mechanisms of plant defenses against stresses and …

Overloaded and stressed: whole-cell considerations for bacterial synthetic biology

O Borkowski, F Ceroni, GB Stan, T Ellis - Current opinion in microbiology, 2016‏ - Elsevier
Highlights•Predictability and robustness are major challenges for synthetic
biology.•Engineering bacteria requires putting synthetic constructs into growing host …

Mitochondrial ATP generation is more proteome efficient than glycolysis

Y Shen, HV Dinh, ER Cruz, Z Chen… - Nature chemical …, 2024‏ - nature.com
Metabolic efficiency profoundly influences organismal fitness. Nonphotosynthetic organisms,
from yeast to mammals, derive usable energy primarily through glycolysis and respiration …

Fecal microbiota transplant promotes response in immunotherapy-refractory melanoma patients

EN Baruch, I Youngster, G Ben-Betzalel, R Ortenberg… - Science, 2021‏ - science.org
The gut microbiome has been shown to influence the response of tumors to anti–PD-1
(programmed cell death–1) immunotherapy in preclinical mouse models and observational …

Targeting cancer with small-molecule pan-KRAS degraders

J Popow, W Farnaby, A Gollner, C Kofink, G Fischer… - Science, 2024‏ - science.org
Mutations in the Kirsten rat sarcoma viral oncogene homolog (KRAS) protein are highly
prevalent in cancer. However, small-molecule concepts that address oncogenic KRAS …

A linked organ-on-chip model of the human neurovascular unit reveals the metabolic coupling of endothelial and neuronal cells

BM Maoz, A Herland, EA FitzGerald, T Grevesse… - Nature …, 2018‏ - nature.com
The neurovascular unit (NVU) regulates metabolic homeostasis as well as drug
pharmacokinetics and pharmacodynamics in the central nervous system. Metabolic fluxes …

Stringent response ensures the timely adaptation of bacterial growth to nutrient downshift

M Zhu, X Dai - Nature Communications, 2023‏ - nature.com
Timely adaptation to nutrient downshift is crucial for bacteria to maintain fitness during feast
and famine cycle in the natural niche. However, the molecular mechanism that ensures the …

Proteomics of melanoma response to immunotherapy reveals mitochondrial dependence

M Harel, R Ortenberg, SK Varanasi, KC Mangalhara… - Cell, 2019‏ - cell.com
Immunotherapy has revolutionized cancer treatment, yet most patients do not respond. Here,
we investigated mechanisms of response by profiling the proteome of clinical samples from …

Metabolite concentrations, fluxes and free energies imply efficient enzyme usage

JO Park, SA Rubin, YF Xu, D Amador-Noguez… - Nature chemical …, 2016‏ - nature.com
In metabolism, available free energy is limited and must be divided across pathway steps to
maintain a negative Δ G throughout. For each reaction, Δ G is log proportional both to a …

Global characterization of in vivo enzyme catalytic rates and their correspondence to in vitro kcat measurements

D Davidi, E Noor, W Liebermeister, A Bar-Even… - Proceedings of the …, 2016‏ - pnas.org
Turnover numbers, also known as k cat values, are fundamental properties of enzymes.
However, k cat data are scarce and measured in vitro, thus may not faithfully represent the in …