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Advances in giant unilamellar vesicle preparation techniques and applications
Giant unilamellar vesicles (GUVs) are versatile and promising cell-sized bio-membrane
mimetic platforms. Their applications range from understanding and quantifying membrane …
mimetic platforms. Their applications range from understanding and quantifying membrane …
Giant unilamellar vesicle electroformation: What to use, what to avoid, and how to quantify the results
Since its inception more than thirty years ago, electroformation has become the most
commonly used method for growing giant unilamellar vesicles (GUVs). Although the method …
commonly used method for growing giant unilamellar vesicles (GUVs). Although the method …
Artificial cells drive neural differentiation
We report the construction of artificial cells that chemically communicate with mammalian
cells under physiological conditions. The artificial cells respond to the presence of a small …
cells under physiological conditions. The artificial cells respond to the presence of a small …
Artificial DNA framework channel modulates antiapoptotic behavior in ischemia-stressed cells via destabilizing promoter G-quadruplex
X Han, S Xu, L Wang, Z Bi, D Wang, H Bu, J Da, Y Liu… - ACS …, 2024 - ACS Publications
Regulating folding/unfolding of gene promoter G-quadruplexes (G4s) is important for
understanding the topological changes in genomic DNAs and the biological effects of such …
understanding the topological changes in genomic DNAs and the biological effects of such …
A practical guide to preparation and applications of giant unilamellar vesicles formed via centrifugation of water-in-oil emulsion droplets
Giant vesicles (GVs), which are closed lipid bilayer membranes with a diameter of more than
1 μm, have attracted attention not only as model cell membranes but also for the …
1 μm, have attracted attention not only as model cell membranes but also for the …
Current problems and future avenues in proteoliposome research
AM Amati, S Graf, S Deutschmann… - Biochemical Society …, 2020 - portlandpress.com
Membrane proteins (MPs) are the gatekeepers between different biological compartments
separated by lipid bilayers. Being receptors, channels, transporters, or primary pumps, they …
separated by lipid bilayers. Being receptors, channels, transporters, or primary pumps, they …
Lipid giant vesicles engulf living bacteria triggered by minor enhancement in membrane fluidity
S Dai, X Tang, N Zhang, H Li, C He, Y Han, Y Wang - Nano letters, 2022 - ACS Publications
Antibacterial amphiphiles normally kill bacteria by destroying the bacterial membrane.
Whether and how antibacterial amphiphiles alter normal cell membrane and lead to …
Whether and how antibacterial amphiphiles alter normal cell membrane and lead to …
Imaging flow cytometry for high-throughput phenoty** of synthetic cells
E Godino, AM Restrepo Sierra… - ACS Synthetic Biology, 2023 - ACS Publications
The reconstitution of basic cellular functions in micrometer-sized liposomes has led to a
surge of interest in the construction of synthetic cells. Microscopy and flow cytometry are …
surge of interest in the construction of synthetic cells. Microscopy and flow cytometry are …
Application of flow cytometry as novel technology in studying the effect of droplet size on lipid oxidation in oil-in-water emulsions
Despite several published studies, the impact of emulsion droplet size on lipid oxidation
rates is unclear. This could be because oil-in-water emulsions are typically polydisperse and …
rates is unclear. This could be because oil-in-water emulsions are typically polydisperse and …
Growth of giant peptide vesicles driven by compartmentalized transcription–translation activity
Compartmentalization and spatial organization of biochemical reactions are essential for the
establishment of complex metabolic pathways inside synthetic cells. Phospholipid and fatty …
establishment of complex metabolic pathways inside synthetic cells. Phospholipid and fatty …