Advances in giant unilamellar vesicle preparation techniques and applications

KS Nair, H Bajaj - Advances in Colloid and Interface Science, 2023 - Elsevier
Giant unilamellar vesicles (GUVs) are versatile and promising cell-sized bio-membrane
mimetic platforms. Their applications range from understanding and quantifying membrane …

Giant unilamellar vesicle electroformation: What to use, what to avoid, and how to quantify the results

Z Boban, I Mardešić, WK Subczynski, M Raguz - Membranes, 2021 - mdpi.com
Since its inception more than thirty years ago, electroformation has become the most
commonly used method for growing giant unilamellar vesicles (GUVs). Although the method …

Artificial cells drive neural differentiation

ÖD Toparlak, J Zasso, S Bridi, MD Serra, P Macchi… - Science …, 2020 - science.org
We report the construction of artificial cells that chemically communicate with mammalian
cells under physiological conditions. The artificial cells respond to the presence of a small …

Artificial DNA framework channel modulates antiapoptotic behavior in ischemia-stressed cells via destabilizing promoter G-quadruplex

X Han, S Xu, L Wang, Z Bi, D Wang, H Bu, J Da, Y Liu… - ACS …, 2024 - ACS Publications
Regulating folding/unfolding of gene promoter G-quadruplexes (G4s) is important for
understanding the topological changes in genomic DNAs and the biological effects of such …

A practical guide to preparation and applications of giant unilamellar vesicles formed via centrifugation of water-in-oil emulsion droplets

Y Zhang, H Obuchi, T Toyota - Membranes, 2023 - mdpi.com
Giant vesicles (GVs), which are closed lipid bilayer membranes with a diameter of more than
1 μm, have attracted attention not only as model cell membranes but also for the …

Current problems and future avenues in proteoliposome research

AM Amati, S Graf, S Deutschmann… - Biochemical Society …, 2020 - portlandpress.com
Membrane proteins (MPs) are the gatekeepers between different biological compartments
separated by lipid bilayers. Being receptors, channels, transporters, or primary pumps, they …

Lipid giant vesicles engulf living bacteria triggered by minor enhancement in membrane fluidity

S Dai, X Tang, N Zhang, H Li, C He, Y Han, Y Wang - Nano letters, 2022 - ACS Publications
Antibacterial amphiphiles normally kill bacteria by destroying the bacterial membrane.
Whether and how antibacterial amphiphiles alter normal cell membrane and lead to …

Imaging flow cytometry for high-throughput phenoty** of synthetic cells

E Godino, AM Restrepo Sierra… - ACS Synthetic Biology, 2023 - ACS Publications
The reconstitution of basic cellular functions in micrometer-sized liposomes has led to a
surge of interest in the construction of synthetic cells. Microscopy and flow cytometry are …

Application of flow cytometry as novel technology in studying the effect of droplet size on lipid oxidation in oil-in-water emulsions

P Li, DJ McClements, EA Decker - Journal of Agricultural and …, 2019 - ACS Publications
Despite several published studies, the impact of emulsion droplet size on lipid oxidation
rates is unclear. This could be because oil-in-water emulsions are typically polydisperse and …

Growth of giant peptide vesicles driven by compartmentalized transcription–translation activity

T Frank, K Vogele, A Dupin… - … –A European Journal, 2020 - Wiley Online Library
Compartmentalization and spatial organization of biochemical reactions are essential for the
establishment of complex metabolic pathways inside synthetic cells. Phospholipid and fatty …