Advances in microfluidic PCR for point-of-care infectious disease diagnostics

S Park, Y Zhang, S Lin, TH Wang, S Yang - Biotechnology advances, 2011‏ - Elsevier
Global burdens from existing or emerging infectious diseases emphasize the need for point-
of-care (POC) diagnostics to enhance timely recognition and intervention. Molecular …

Diagnosis of bloodstream infections: an evolution of technologies towards accurate and rapid identification and antibiotic susceptibility testing

KC Tjandra, N Ram-Mohan, R Abe, MM Hashemi… - Antibiotics, 2022‏ - mdpi.com
Bloodstream infections (BSI) are a leading cause of death worldwide. The lack of timely and
reliable diagnostic practices is an ongoing issue for managing BSI. The current gold …

[HTML][HTML] Next-generation molecular diagnostics: Leveraging digital technologies to enhance multiplexing in real-time PCR

L Kreitmann, L Miglietta, K Xu… - TrAC Trends in …, 2023‏ - Elsevier
Real-time polymerase chain reaction (qPCR) enables accurate detection and quantification
of nucleic acids and has become a fundamental tool in biological sciences, bioengineering …

Catheterization alters bladder ecology to potentiate Staphylococcus aureus infection of the urinary tract

JN Walker, AL Flores-Mireles, CL Pinkner… - Proceedings of the …, 2017‏ - pnas.org
Methicillin-resistant Staphylococcus aureus (MRSA) is an emerging cause of catheter-
associated urinary tract infection (CAUTI), which frequently progresses to more serious …

High-resolution DNA melting analysis in clinical research and diagnostics

JL Montgomery, LN Sanford… - Expert review of molecular …, 2010‏ - Taylor & Francis
Among nucleic acid analytical methods, high-resolution melting analysis is gaining more
and more attention. High-resolution melting provides simple, homogeneous solutions for …

Microbiological applications of high-resolution melting analysis

SYC Tong, PM Giffard - Journal of clinical microbiology, 2012‏ - journals.asm.org
High-resolution melting (HRM) analysis uses real-time PCR instrumentation to interrogate
DNA sequence variation and is a low-cost, single-step, closed-tube method. Here we …

Targeted treatment for bacterial infections: prospects for pathogen-specific antibiotics coupled with rapid diagnostics

T Maxson, DA Mitchell - Tetrahedron, 2016‏ - Elsevier
The advent of antibiotics in the early twentieth century catalyzed a medical revolution,
drastically reducing mortality due to bacterial infections. Along with numerous other …

Nanoarray digital polymerase chain reaction with high-resolution melt for enabling broad bacteria identification and pheno–molecular antimicrobial susceptibility test

P Athamanolap, K Hsieh, CM O'Keefe… - Analytical …, 2019‏ - ACS Publications
Toward combating infectious diseases caused by pathogenic bacteria, there remains an
unmet need for diagnostic tools that can broadly identify the causative bacteria and …

Integrated bacterial identification and antimicrobial susceptibility testing using PCR and high-resolution melt

P Athamanolap, K Hsieh, L Chen, S Yang… - Analytical …, 2017‏ - ACS Publications
Accurate and timely diagnostics are critical for managing bacterial infections. The current
gold standard, culture-based diagnostics, can provide clinicians with comprehensive …

Emerging analytical techniques for rapid pathogen identification and susceptibility testing

DJ Shin, N Andini, K Hsieh, S Yang… - Annual review of …, 2019‏ - annualreviews.org
In the face of looming threats from multi-drug resistant microorganisms, there is a growing
need for technologies that will enable rapid identification and drug susceptibility profiling of …