In the era of quantitative proteomics, where mass spectrometry plays a pivotal role, in
particular associated with the use of data‐independent acquisition, it is time to perform an …

High-throughput lysis and proteolytic digestion of biopsy-level tissue specimens is a major
bottleneck for clinical proteomics. Here we describe a detailed protocol of pressure cycling …

Efficient and reproducible sample preparation is a prerequisite for any robust and sensitive
quantitative bottom-up proteomics workflow. Here, we performed an independent …

Formalin‐fixed, paraffin‐embedded (FFPE), biobanked tissue samples offer an invaluable
resource for clinical and biomarker research. Here, we developed a pressure cycling …

Determination of malignancy in thyroid nodules remains a major diagnostic challenge. Here
we report the feasibility and clinical utility of develo** an AI-defined protein-based …

Pressure cycling technology (PCT)-assisted tissue lysis and digestion have facilitated
reproducible and high-throughput proteomic studies of both fresh-frozen (FF) and formalin …

Here we describe a proteomic data resource for the NCI-60 cell lines generated by pressure
cycling technology and SWATH mass spectrometry. We developed the DIA-expert software …

Tissues and biofluids are important sources of information used for the detection of diseases
and decisions on patient therapies. There are several accepted methods for preservation of …

The human body comprises rich populations of cells, which are arranged into tissues and
organs with diverse functionalities. These cells exhibit a broad spectrum of phenotypes and …

Coenzyme A (CoA) and acetyl-coenzyme A (acetyl-CoA) play essential roles in cell energy
metabolism. Dysregulation of the biosynthesis and functioning of both compounds may …