Biotechnological domestication of pseudomonads using synthetic biology
Much of contemporary synthetic biology research relies on the use of bacterial chassis for
plugging-in and plugging-out genetic circuits and new-to-nature functionalities. However …
plugging-in and plugging-out genetic circuits and new-to-nature functionalities. However …
[HTML][HTML] A navigation guide of synthetic biology tools for Pseudomonas putida
Pseudomonas putida is a microbial chassis of huge potential for industrial and
environmental biotechnology, owing to its remarkable metabolic versatility and ability to …
environmental biotechnology, owing to its remarkable metabolic versatility and ability to …
Pseudomonas putida KT2440 strain metabolizes glucose through a cycle formed by enzymes of the Entner-Doudoroff, Embden-Meyerhof-Parnas, and pentose …
The soil bacterium Pseudomonas putida KT2440 lacks a functional Embden-Meyerhof-
Parnas (EMP) pathway, and glycolysis is known to proceed almost exclusively through the …
Parnas (EMP) pathway, and glycolysis is known to proceed almost exclusively through the …
Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress
As a frequent inhabitant of sites polluted with toxic chemicals, the soil bacterium and plant-
root colonizer Pseudomonas putida can tolerate high levels of endogenous and exogenous …
root colonizer Pseudomonas putida can tolerate high levels of endogenous and exogenous …
High‐quality genome‐scale metabolic modelling of Pseudomonas putida highlights its broad metabolic capabilities
Genome‐scale reconstructions of metabolism are computational species‐specific
knowledge bases able to compute systemic metabolic properties. We present a …
knowledge bases able to compute systemic metabolic properties. We present a …
Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression
Background Because of its adaptability to sites polluted with toxic chemicals, the model soil
bacterium Pseudomonas putida is naturally endowed with a number of metabolic and stress …
bacterium Pseudomonas putida is naturally endowed with a number of metabolic and stress …
The Entner–Doudoroff pathway empowers Pseudomonas putida KT2440 with a high tolerance to oxidative stress
Glucose catabolism of P seudomonas putida is carried out exclusively through the E ntner–
D oudoroff (ED) pathway due to the absence of 6‐phosphofructokinase. In order to activate …
D oudoroff (ED) pathway due to the absence of 6‐phosphofructokinase. In order to activate …
[HTML][HTML] Model-guided dynamic control of essential metabolic nodes boosts acetyl-coenzyme A–dependent bioproduction in rewired Pseudomonas putida
Pseudomonas putida is evolutionarily endowed with features relevant for bioproduction,
especially under harsh operating conditions. The rich metabolic versatility of this species …
especially under harsh operating conditions. The rich metabolic versatility of this species …
[HTML][HTML] GC-MS-based 13C metabolic flux analysis resolves the parallel and cyclic glucose metabolism of Pseudomonas putida KT2440 and Pseudomonas …
The genus Pseudomonas comprises approximately 200 species with numerous isolates that
are common inhabitants of soil, water, and vegetation and has been of particular interest for …
are common inhabitants of soil, water, and vegetation and has been of particular interest for …
The metabolic cost of flagellar motion in Pseudomonas putida KT2440
Although the flagellar machinery of environmental bacteria endows cells with a phenomenal
survival device, it also consumes much of the metabolic currency necessary for fuelling such …
survival device, it also consumes much of the metabolic currency necessary for fuelling such …