Activity-based protein profiling (ABPP) is a technique that uses highly selective active-site
targeted chemical probes to label and monitor the state of proteins. ABPP integrates the …

Proteasomes are large, multicatalytic protein complexes that cleave cellular proteins into
peptides. There are many distinct forms of proteasomes that differ in catalytically active …

The selective degradation of many short-lived proteins in eukaryotic cells is carried out by
the ubiquitin system. In this pathway, proteins are targeted for degradation by covalent …

AAA+ family proteolytic machines (ClpXP, ClpAP, ClpCP, HslUV, Lon, FtsH, PAN/20S, and
the 26S proteasome) perform protein quality control and are used in regulatory circuits in all …

Proteasomes are large, multisubunit proteolytic complexes presenting multiple targets for
therapeutic intervention. The 26S proteasome consists of a 20S proteolytic core and one or …

▪ Abstract In eukaryotic cells, most proteins in the cytosol and nucleus are degraded via the
ubiquitin-proteasome pathway. The 26S proteasome is a 2.5-MDa molecular machine built …

With the postgenome era rapidly approaching, new strategies for the functional analysis of
proteins are needed. To date, proteomics efforts have primarily been confined to recording …

Proteasomes are major sites for protein degradation in eukaryotic cells. The recent
identification of selective proteasome inhibitors has allowed a definition of the roles of the …

Proteases or proteolytic enzymes form one of the largest and more important groups of
enzymes. Proteases selectively catalyze the hydrolysis of peptide bonds and can be divided …

Arylfluorosulfates have appeared only rarely in the literature and have not been explored as
probes for covalent conjugation to proteins, possibly because they were assumed to …