Metabolism of xenobiotics remains a central challenge for the discovery and development of
drugs, cosmetics, nutritional supplements, and agrochemicals. Metabolic transformations …

Cooperative functional properties and allosteric regulation in cytochromes P450 play an
important role in xenobiotic metabolism and define one of the main mechanisms of drug …

Proteins exist as ensembles of interconverting states on a complex energy landscape. A
complete, molecular-level understanding of their function requires knowledge of the …

Cytochrome P450 monooxygenases typically catalyze the insertion of one atom of oxygen
from O2 into unactivated carbon-hydrogen and carbon-carbon bonds, with concomitant …

The human steroidogenic cytochrome P450 CYP17A1 catalyzes two types of reactions in
the biosynthetic pathway leading from pregnenolone to testosterone and several other …

Conspectus Enzyme function requires that enzyme structures be dynamic. Substrate
binding, product release, and transition state stabilization typically involve different enzyme …

Carbon monoxide (CO) is a product of haem metabolism and organisms must evolve
strategies to prevent endogenous CO poisoning of haemoproteins. We show that energy …

Many economically important biosyntheses incorporate regiospecific and stereospecific
oxidations at unactivated carbons. Such oxidations are commonly catalyzed by cytochrome …

The broad and variable substrate specificity of cytochrome P450 enzymes makes them a
model system for studying the determinants of protein molecular recognition. The archetypal …

Abstract CYP106A2 (cytochrome P450 meg) is a bacterial enzyme originally isolated from B.
megaterium, and has been shown to hydroxylate a wide variety of substrates, including …