Enzymes are efficient and specific catalysts for many essential reactions in biotechnological
and pharmaceutical industries. Many times, the natural enzymes do not display the catalytic …

Directed evolution has emerged as a powerful strategy to engineer various properties of
proteins. Traditional methods to construct libraries such as error-prone PCR and DNA …

We introduce a computational toolset, named GROmaρs, to obtain and compare time-
averaged density maps from molecular dynamics simulations. GROmaρs efficiently …

Kalium channelrhodopsin 1 from Hyphochytrium catenoides (Hc KCR1) is a light-gated
channel used for optogenetic silencing of mammalian neurons. It selects K+ over Na+ in the …

Threonine deaminase (TD, EC 4.3. 1.19) mediates α, β-elimination (deamination), which has
untapped potential in the synthesis of unnatural α-keto acids. However, the narrow substrate …

Engineering microenvironments for sequential enzymatic reactions has attracted specific
interest within different fields of research as an effective strategy to improve the catalytic …

Tripartite resistance-nodulation-division (RND) efflux pumps, such as AcrAB-TolC of
Salmonella Typhimurium, contribute to antibiotic resistance and comprise an inner …

Abstract Undoubtedly, the SARS-CoV-2 has become a major concern for all societies due to
its catastrophic effects on public health. In addition, mutations and changes in the structure …

Structural information on channelrhodopsins' mechanism of light-gated ion conductance is
scarce, limiting its engineering as optogenetic tools. Here, we use single-particle cryo …

Viperin is an important virus-induced protein in animals that negatively participates in RNA
viral replication and transcription. The reactive machinery of viperin suggests that it …