Cell–cell interactions orchestrate organismal development, homeostasis and single-cell
functions. When cells do not properly interact or improperly decode molecular messages …

Gene expression involves transcription, translation and the turnover of mRNAs and proteins.
The degree to which protein abundances scale with mRNA levels and the implications in …

Improved scale, multiplexing and resolution are establishing spatial nucleic acid and protein
profiling methods as a major pillar for cellular atlas building of complex samples, from …

Alternative splicing is a substantial contributor to the high complexity of transcriptomes of
multicellular eukaryotes. In this Review, we discuss the accumulated evidence that most of …

In the last decade, single-cell RNA sequencing routinely performed on large numbers of
single cells has greatly advanced our understanding of the underlying heterogeneity of …

Proteins are the main targets of most drugs; however, system-wide methods to monitor
protein activity and function are still underused in drug discovery. Novel biochemical …

INTRODUCTION Many complex human phenotypes, including diseases, exhibit sex-
differentiated characteristics. These sex differences have been variously attributed to …

The paired measurement of RNA and surface proteins in single cells with cellular indexing
of transcriptomes and epitopes by sequencing (CITE-seq) is a promising approach to …

Chemotherapy, radiation therapy, as well as targeted anticancer agents can induce clinically
relevant tumor-targeting immune responses, which critically rely on the antigenicity of …

Proteins are essential agents of biological processes. To date, large-scale profiling of cell
line collections including the Cancer Cell Line Encyclopedia (CCLE) has focused primarily …