Response of plants to water stress
Water stress adversely impacts many aspects of the physiology of plants, especially
photosynthetic capacity. If the stress is prolonged, plant growth, and productivity are severely …
photosynthetic capacity. If the stress is prolonged, plant growth, and productivity are severely …
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs)
comprise a powerful class of tools that are redefining the boundaries of biological research …
comprise a powerful class of tools that are redefining the boundaries of biological research …
Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
Substantial efforts are being made to optimize the CRISPR/Cas9 system for precision crop
breeding. The avoidance of transgene integration and reduction of off-target mutations are …
breeding. The avoidance of transgene integration and reduction of off-target mutations are …
A highly specific SpCas9 variant is identified by in vivo screening in yeast
Despite the utility of CRISPR–Cas9 nucleases for genome editing, the potential for off-target
activity limits their application, especially for therapeutic purposes,. We developed a yeast …
activity limits their application, especially for therapeutic purposes,. We developed a yeast …
A multipurpose toolkit to enable advanced genome engineering in plants
We report a comprehensive toolkit that enables targeted, specific modification of monocot
and dicot genomes using a variety of genome engineering approaches. Our reagents …
and dicot genomes using a variety of genome engineering approaches. Our reagents …
Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew
Sequence-specific nucleases have been applied to engineer targeted modifications in
polyploid genomes, but simultaneous modification of multiple homoeoalleles has not been …
polyploid genomes, but simultaneous modification of multiple homoeoalleles has not been …
A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation
The relative ease, speed, and biological scope of clustered regularly interspaced short
palindromic repeats (CRISPR)/CRISPR-associated Protein9 (Cas9)-based reagents for …
palindromic repeats (CRISPR)/CRISPR-associated Protein9 (Cas9)-based reagents for …
Targeted genome modification of crop plants using a CRISPR-Cas system
To the Editor: Although genome editing technologies using zinc finger nucleases (ZFNs) 1
and transcription activator-like effector nucleases (TALENs) 2 can generate genome …
and transcription activator-like effector nucleases (TALENs) 2 can generate genome …
[HTML][HTML] CRISPR/Cas9 platforms for genome editing in plants: developments and applications
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein9
(Cas9) genome editing system (CRISPR/Cas9) is adapted from the prokaryotic type II …
(Cas9) genome editing system (CRISPR/Cas9) is adapted from the prokaryotic type II …
Genome editing in rice and wheat using the CRISPR/Cas system
Targeted genome editing nucleases, such as zinc-finger nucleases (ZFNs) and transcription
activator-like effector nucleases (TALENs), are powerful tools for understanding gene …
activator-like effector nucleases (TALENs), are powerful tools for understanding gene …