New technologies for analysis of extracellular vesicles

H Shao, H Im, CM Castro, X Breakefield… - Chemical …, 2018 - ACS Publications
Extracellular vesicles (EVs) are diverse, nanoscale membrane vesicles actively released by
cells. Similar-sized vesicles can be further classified (eg, exosomes, microvesicles) based …

Technical challenges of working with extracellular vesicles

MI Ramirez, MG Amorim, C Gadelha, I Milic, JA Welsh… - Nanoscale, 2018 - pubs.rsc.org
Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with
potential applications in diagnosis, prognosis and therapeutic guidance in most pathological …

LncRNA RPPH1 promotes colorectal cancer metastasis by interacting with TUBB3 and by promoting exosomes-mediated macrophage M2 polarization

Z Liang, H Liu, F Wang, L **ong, C Zhou, T Hu… - Cell death & …, 2019 - nature.com
Metastasis is a well-known poor prognostic factor in cancer. However, the mechanisms how
long non-coding RNAs (lncRNAs) regulate metastasis in colorectal cancer (CRC) remain …

Coding and noncoding landscape of extracellular RNA released by human glioma stem cells

Z Wei, AO Batagov, S Schinelli, J Wang, Y Wang… - Nature …, 2017 - nature.com
Tumor-released RNA may mediate intercellular communication and serve as biomarkers.
Here we develop a protocol enabling quantitative, minimally biased analysis of extracellular …

[HTML][HTML] Translating RNA sequencing into clinical diagnostics: opportunities and challenges

SA Byron, KR Van Keuren-Jensen… - Nature Reviews …, 2016 - nature.com
With the emergence of RNA sequencing (RNA-seq) technologies, RNA-based biomolecules
hold expanded promise for their diagnostic, prognostic and therapeutic applicability in …

A comparative study of serum exosome isolation using differential ultracentrifugation and three commercial reagents

I Helwa, J Cai, MD Drewry, A Zimmerman, MB Dinkins… - PloS one, 2017 - journals.plos.org
Exosomes play a role in cell-to-cell signaling and serve as possible biomarkers. Isolating
exosomes with reliable quality and substantial concentration is a major challenge. Our …

MVP-mediated exosomal sorting of miR-193a promotes colon cancer progression

Y Teng, Y Ren, X Hu, J Mu, A Samykutty… - Nature …, 2017 - nature.com
Exosomes are emerging mediators of intercellular communication; whether the release of
exosomes has an effect on the exosome donor cells in addition to the recipient cells has not …

[HTML][HTML] Exosomal long noncoding RNA CRNDE-h as a novel serum-based biomarker for diagnosis and prognosis of colorectal cancer

T Liu, X Zhang, S Gao, F **g, Y Yang, L Du, G Zheng… - Oncotarget, 2016 - ncbi.nlm.nih.gov
Cancer-secreted long non-coding RNAs (lncRNAs) are emerging mediators of cancer-host
cross talk. The aim of our study was to illustrate the clinical significance of the lncRNA …

Altered extracellular vesicle concentration, cargo, and function in diabetes

DW Freeman, N Noren Hooten, E Eitan, J Green… - Diabetes, 2018 - Am Diabetes Assoc
Type 2 diabetes is a chronic age-associated degenerative metabolic disease that reflects
relative insulin deficiency and resistance. Extracellular vesicles (EVs)(exosomes …

The extracellular RNA communication consortium: establishing foundational knowledge and technologies for extracellular RNA research

S Das, AB Abdel-Mageed, C Adamidi, PD Adelson… - Cell, 2019 - cell.com
The Extracellular RNA Communication Consortium (ERCC) was launched to accelerate
progress in the new field of extracellular RNA (exRNA) biology and to establish whether …