Site-selective orientated immobilization of antibodies and conjugates for immunodiagnostics development

M Shen, JF Rusling, CK Dixit - Methods, 2017 - Elsevier
Immobilized antibody systems are the key to develop efficient diagnostics and separations
tools. In the last decade, developments in the field of biomolecular engineering and …

[HTML][HTML] Factors influencing properties of spider silk coatings and their interactions within a biological environment

VT Trossmann, S Lentz, T Scheibel - Journal of Functional Biomaterials, 2023 - mdpi.com
Biomaterials are an indispensable part of biomedical research. However, although many
materials display suitable application-specific properties, they provide only poor …

Genetic Functionalization of Protein-Based Biomaterials via Protein Fusions

GG Mendes, B Faulk, B Kaparthi, AR Irion… - …, 2024 - ACS Publications
Proteins implement many useful functions, including binding ligands with unparalleled
affinity and specificity, catalyzing stereospecific chemical reactions, and directing cell …

Self-assembly of recombinant silk as a strategy for chemical-free formation of bioactive coatings: A real-time study

L Nilebäck, J Hedin, M Widhe, LS Floderus… - …, 2017 - ACS Publications
Functionalization of biomaterials with biologically active peptides can improve their
performance after implantation. By genetic fusion to self-assembling proteins, the functional …

Recombinant protein polymer-antibody conjugates for applications in nanotechnology and biomedicine

AG Goncalves, EJ Hartzell, MO Sullivan… - Advanced Drug Delivery …, 2022 - Elsevier
Currently, there are over 100 antibody-based therapeutics on the market for the treatment of
various diseases. The increasing importance of antibody treatment is further highlighted by …

Bioselectivity of silk protein-based materials and their bio-inspired applications

H Bargel, VT Trossmann, C Sommer… - Beilstein Journal of …, 2022 - beilstein-journals.org
Adhesion to material surfaces is crucial for almost all organisms regarding subsequent
biological responses. Mammalian cell attachment to a surrounding biological matrix is …

Silk–silk interactions between silkworm fibroin and recombinant spider silk fusion proteins enable the construction of bioactive materials

L Nileback, D Chouhan, R Jansson… - … Applied Materials & …, 2017 - ACS Publications
Natural silk is easily accessible from silkworms and can be processed into different formats
suitable as biomaterials and cell culture matrixes. Recombinant DNA technology enables …

Silk materials functionalized via genetic engineering for biomedical applications

T Deptuch, H Dams-Kozlowska - Materials, 2017 - mdpi.com
The great mechanical properties, biocompatibility and biodegradability of silk-based
materials make them applicable to the biomedical field. Genetic engineering enables the …

Bioactivation of spider silk with basic fibroblast growth factor for in vitro cell culture: A step toward creation of artificial ECM

N Thatikonda, L Nilebäck, A Kempe… - ACS Biomaterials …, 2018 - ACS Publications
Presentation of immobilized growth factors with retained bioactivity remains a challenge in
the field of tissue engineering. In the present study, we propose a strategy to covalently …

Genetically engineered mucoadhesive spider silk

G Petrou, R Jansson, M Högqvist… - …, 2018 - ACS Publications
Mucoadhesion is defined as the adhesion of a material to the mucus gel covering the
mucous membranes. The mechanisms controlling mucoadhesion include nonspecific …