The central dogma of modern biology underscores the pivotal roles proteins play in diverse
biological processes, the study of which necessitates advanced methods to produce …

The native chemical ligation reaction (NCL) involves reacting a C-terminal peptide thioester
with an N-terminal cysteinyl peptide to produce a native peptide bond between the two …

Protecting group chemistry for the cysteine thiol group has enabled a vast array of peptide
and protein chemistry over the last several decades. Increasingly sophisticated strategies for …

Solid phase peptide synthesis (SPPS) provides the possibility to chemically synthesize
peptides and proteins. Applying the method on hydrophilic structures is usually without …

Nature has developed a plethora of protein machinery to operate and maintain nearly every
task of cellular life. These processes are tightly regulated via post‐expression modifications …

Highly effective yet chemoselective chemical transformation strategies enable the facile
access and precise modification of complicated biomacromolecules. In particular, the …

Activity‐based E2 conjugating enzyme (E2)‐ubiquitin (Ub) probes have recently emerged as
effective tools for studying the molecular mechanism of E3 ligase (E3)‐catalyzed …

Herein we introduce vinylphosphonothiolates as a new class of cysteine-selective
electrophiles for protein labeling and the formation of stable protein–protein conjugates. We …

Site‐selective functionalization strategies are in high demand to prepare well‐defined
homogeneous proteins for basic research and biomedical applications. In this regard …

Histone ubiquitination affects the structure and function of nucleosomes through tightly
regulated dynamic reversible processes. The efficient preparation of ubiquitinated histones …