The technological landscape and applications of single-cell multi-omics

A Baysoy, Z Bai, R Satija, R Fan - Nature Reviews Molecular Cell …, 2023 - nature.com
Single-cell multi-omics technologies and methods characterize cell states and activities by
simultaneously integrating various single-modality omics methods that profile the …

Characterizing cis-regulatory elements using single-cell epigenomics

S Preissl, KJ Gaulton, B Ren - Nature Reviews Genetics, 2023 - nature.com
Cell type-specific gene expression patterns and dynamics during development or in disease
are controlled by cis-regulatory elements (CREs), such as promoters and enhancers …

[HTML][HTML] Organization of the human intestine at single-cell resolution

JW Hickey, WR Becker, SA Nevins, A Horning… - Nature, 2023 - nature.com
The intestine is a complex organ that promotes digestion, extracts nutrients, participates in
immune surveillance, maintains critical symbiotic relationships with microbiota and affects …

Dictionary learning for integrative, multimodal and scalable single-cell analysis

Y Hao, T Stuart, MH Kowalski, S Choudhary… - Nature …, 2024 - nature.com
Map** single-cell sequencing profiles to comprehensive reference datasets provides a
powerful alternative to unsupervised analysis. However, most reference datasets are …

Chemical reprogramming of human somatic cells to pluripotent stem cells

J Guan, G Wang, J Wang, Z Zhang, Y Fu, L Cheng… - Nature, 2022 - nature.com
Cellular reprogramming can manipulate the identity of cells to generate the desired cell
types,–. The use of cell intrinsic components, including oocyte cytoplasm and transcription …

Neutrophils and emergency granulopoiesis drive immune suppression and an extreme response endotype during sepsis

AJ Kwok, A Allcock, RC Ferreira, E Cano-Gamez… - Nature …, 2023 - nature.com
Sepsis arises from diverse and incompletely understood dysregulated host response
processes following infection that leads to life-threatening organ dysfunction. Here we …

Single-cell multi-omics identifies chronic inflammation as a driver of TP53-mutant leukemic evolution

A Rodriguez-Meira, R Norfo, S Wen, AL Chédeville… - Nature …, 2023 - nature.com
Understanding the genetic and nongenetic determinants of tumor protein 53 (TP53)-
mutation-driven clonal evolution and subsequent transformation is a crucial step toward the …

Single-cell analyses define a continuum of cell state and composition changes in the malignant transformation of polyps to colorectal cancer

WR Becker, SA Nevins, DC Chen, R Chiu… - Nature …, 2022 - nature.com
To chart cell composition and cell state changes that occur during the transformation of
healthy colon to precancerous adenomas to colorectal cancer (CRC), we generated single …

Genome-wide enhancer maps link risk variants to disease genes

J Nasser, DT Bergman, CP Fulco, P Guckelberger… - Nature, 2021 - nature.com
Genome-wide association studies (GWAS) have identified thousands of noncoding loci that
are associated with human diseases and complex traits, each of which could reveal insights …

Scalable, multimodal profiling of chromatin accessibility, gene expression and protein levels in single cells

EP Mimitou, CA Lareau, KY Chen… - Nature …, 2021 - nature.com
Recent technological advances have enabled massively parallel chromatin profiling with
scATAC-seq (single-cell assay for transposase accessible chromatin by sequencing). Here …