Structural basis of human separase regulation by securin and CDK1–cyclin B1
In early mitosis, the duplicated chromosomes are held together by the ring-shaped cohesin
complex. Separation of chromosomes during anaphase is triggered by separase—a large …
complex. Separation of chromosomes during anaphase is triggered by separase—a large …
Prefusion structure of human cytomegalovirus glycoprotein B and structural basis for membrane fusion
Human cytomegalovirus (HCMV) causes congenital disease with long-term morbidity.
HCMV glycoprotein B (gB) transitions irreversibly from a metastable prefusion to a stable …
HCMV glycoprotein B (gB) transitions irreversibly from a metastable prefusion to a stable …
Cryo-EM grid preparation of membrane protein samples for single particle analysis
Recent advances in cryo-electron microscopy (cryo-EM) have made it possible to solve
structures of biological macromolecules at near atomic resolution. Development of more …
structures of biological macromolecules at near atomic resolution. Development of more …
Molecular structure of the intact bacterial flagellar basal body
The bacterial flagellum is a macromolecular protein complex that enables motility in many
species. Bacterial flagella self-assemble a strong, multicomponent drive shaft that couples …
species. Bacterial flagella self-assemble a strong, multicomponent drive shaft that couples …
Diverse cytomotive actins and tubulins share a polymerization switch mechanism conferring robust dynamics
Protein filaments are used in myriads of ways to organize other molecules within cells. Some
filament-forming proteins couple the hydrolysis of nucleotides to their polymerization cycle …
filament-forming proteins couple the hydrolysis of nucleotides to their polymerization cycle …
In situ and high‐resolution cryo‐EM structure of a bacterial type VI secretion system membrane complex
Bacteria have evolved macromolecular machineries that secrete effectors and toxins to
survive and thrive in diverse environments. The type VI secretion system (T6 SS) is a …
survive and thrive in diverse environments. The type VI secretion system (T6 SS) is a …
Structural basis for assembly of non-canonical small subunits into type IC Cascade
Bacteria and archaea employ CRISPR (clustered, regularly, interspaced, short palindromic
repeats)-Cas (CRISPR-associated) systems as a type of adaptive immunity to target and …
repeats)-Cas (CRISPR-associated) systems as a type of adaptive immunity to target and …
Structural snapshots of R-loop formation by a type IC CRISPR Cascade
Summary Type I CRISPR-Cas systems employ multi-subunit Cascade effector complexes to
target foreign nucleic acids for destruction. Here, we present structures of D. vulgaris type IC …
target foreign nucleic acids for destruction. Here, we present structures of D. vulgaris type IC …
[PDF][PDF] Industrial cryo-EM facility setup and management
K Sader, R Matadeen, P Castro Hartmann… - … Section D: Structural …, 2020 - journals.iucr.org
Cryo-electron microscopy (cryo-EM) has rapidly expanded with the introduction of direct
electron detectors, improved image-processing software and automated image acquisition …
electron detectors, improved image-processing software and automated image acquisition …
The cryo‐EM structure of the BoNT/Wo‐NTNH complex reveals two immunoglobulin‐like domains
The botulinum neurotoxin‐like toxin from Weissella oryzae (BoNT/Wo) is one of the BoNT‐
like toxins recently identified outside of the Clostridium genus. We show that, like the …
like toxins recently identified outside of the Clostridium genus. We show that, like the …