Target-identification and mechanism-of-action studies have important roles in small-
molecule probe and drug discovery. Biological and technological advances have resulted in …

Mass spectrometry (MS) is the most comprehensive and versatile tool in large-scale
proteomics. In this review, we dissect the overall framework of the MS experiment into its key …

Mitochondria are key organelles for cellular energetics, metabolism, signaling, and quality
control and have been linked to various diseases. Different views exist on the composition of …

During eukaryotic evolution, ribosomes have considerably increased in size, forming a
surface-exposed ribosomal RNA (rRNA) shell of unknown function, which may create an …

Lenalidomide is a drug with clinical efficacy in multiple myeloma and other B cell
neoplasms, but its mechanism of action is unknown. Using quantitative proteomics, we …

SILAC is based on direct addition of selected stable isotope amino acids into the cell culture
medium, allowing superior quantitative analysis of the cellular proteome compared to other …

Cellular organelles provide opportunities to relate biological mechanisms to disease. Here
we use affinity proteomics, genetics and cell biology to interrogate cilia: poorly understood …

To elucidate cellular events underlying the pluripotency of human embryonic stem cells
(hESCs), we performed parallel quantitative proteomic and phosphoproteomic analyses of …

It is generally assumed that recurrent mutations within a given cancer driver gene elicit
similar drug responses. Cancer genome studies have identified recurrent but divergent …

Mass spectrometry (MS)-based proteomics is increasingly applied in a quantitative format,
often based on labeling of samples with stable isotopes that are introduced chemically or …