Over the past decades, nitrilases have drawn considerable attention because of their
application in nitrile degradation as prominent biocatalysts. Nitrilases are derived from …

Assaying enzyme-catalyzed transformations in high-throughput is crucial to enzyme
discovery, enzyme engineering and the drug discovery process. In enzyme assays, catalytic …

Background Arginine depletion is a putative target in hepatocellular carcinoma (HCC). HCC
often lacks argininosuccinate synthetase, a citrulline to arginine-repleting enzyme. ADI-PEG …

Nitrilases are widely distributed in nature and are able to hydrolyze nitriles into their
corresponding carboxylic acids and ammonia. In industry, nitrilases have been used as …

Hydrolase‐catalyzed reactions have been widely applied in organic synthesis. Nitrilases are
an important class of hydrolase that converts naturally occurring, as well as xenobiotically …

The objective of the study was to enhance the substrate tolerance of Pseudomonas putida
nitrilase via atmospheric and room temperature plasma (ARTP) and cell immobilization. The …

Iminodiacetic acid (IDA) is widely used as an intermediate in the manufacturing of chelating
agents, glyphosate herbicides and surfactants. To improve activity and tolerance to the …

The highly enantioselective arylacetonitrilase of Pseudomonas putida was purified to
homogeneity using a combination of (NH 4) 2 SO 4 fractionation and different …

Enzyme assays are analytical tools to visualize enzyme activities. In recent years a large
variety of enzyme assays have been developed to assist the discovery and optimization of …

Protein engineering is a powerful tool for improving the properties of enzymes. However,
large changes in enzyme properties are still challenging for traditional evolution strategies …