Single-molecule localization microscopy (SMLM) generates super-resolution images by
serially detecting individual fluorescent molecules. The power of SMLM, however, goes …

Type III protein secretion machines have evolved to deliver bacterially encoded effector
proteins into eukaryotic cells. Although electron microscopy has provided a detailed view of …

Photoactivated localization microscopy (PALM) produces an array of localization
coordinates by means of photoactivatable fluorescent proteins. However, observations are …

Single-molecule localization microscopy (SMLM) relies on the blinking behavior of a
fluorophore, which is the stochastic switching between fluorescent and dark states. Blinking …

Single molecule imaging approaches like dSTORM and PALM resolve structures at 10–20
nm, and allow for unique insights into protein stoichiometry and spatial relationships …

The spatial distribution of proteins within the cell affects their capability to interact with other
molecules and directly influences cellular processes and signaling. At the plasma …

Cell surface protein and lipid molecules are organized in various patterns: randomly, along
gradients, or clustered when segregated into discrete micro-and nano-domains. Their …

Spatial aggregation of proteins might have functional importance, eg, in signaling, and nano-
imaging can be used to study them. Such studies require accurate characterization of …

Clustering of proteins is crucial for many cellular processes and can be imaged at nanoscale
resolution using single-molecule localization microscopy (SMLM). Ideally, molecular …

Ripley's K function was developed to analyze the spatial distribution characteristics in point
pattern analysis, including geography, economics and biomedical research. In biomedical …