Fluorescent Flippers: Small‐Molecule Probes to Image Membrane Tension in Living Systems

XX Chen, F Bayard, N Gonzalez‐Sanchis… - Angewandte …, 2023‏ - Wiley Online Library
Flipper probes have been introduced as small molecule fluorophores to image physical
forces, that is, membrane tension in living systems. Their emergence over one decade is …

Stimuli‐responsive natural proteins and their applications

N Du, F Ye, J Sun, K Liu - ChemBioChem, 2022‏ - Wiley Online Library
Natural proteins are essential biomacromolecules that fulfill versatile functions in the living
organism, such as their usage as cytoskeleton, nutriment transporter, homeostasis …

HydroFlipper membrane tension probes: imaging membrane hydration and mechanical compression simultaneously in living cells

J García-Calvo, J López-Andarias, J Maillard… - Chemical …, 2022‏ - pubs.rsc.org
HydroFlippers are introduced as the first fluorescent membrane tension probes that report
simultaneously on membrane compression and hydration. The probe design is centered …

A tethered ligand assay to probe SARS-CoV-2: ACE2 interactions

MS Bauer, S Gruber, A Hausch, LF Milles, T Nicolaus… - Biophysical …, 2022‏ - cell.com
SARS-CoV-2 infections are initiated by attachment of the viral receptor-binding domain
(RBD) to angiotensin-converting enzyme-2 (ACE2) on human host cells. This critical first …

Engineering photoresponsive ligand tethers for mechanical regulation of stem cells

J Zhang, SHD Wong, X Wu, H Lei, M Qin… - Advanced …, 2021‏ - Wiley Online Library
Regulating stem cell functions by precisely controlling the nanoscale presentation of
bioactive ligands has a substantial impact on tissue engineering and regenerative medicine …

Histidine-specific bioconjugation for single-molecule force spectroscopy

H Lei, J Zhang, Y Li, X Wang, M Qin, W Wang, Y Cao - ACS nano, 2022‏ - ACS Publications
Atomic force microscopy (AFM) based single-molecule force spectroscopy (SMFS) is a
powerful tool to study the mechanical properties of proteins. In these experiments, site …

Fluorescent membrane tension probes for early endosomes

F Piazzolla, V Mercier, L Assies, N Sakai… - Angewandte …, 2021‏ - Wiley Online Library
Fluorescent flipper probes have been introduced recently to image membrane tension in live
cells, and strategies to target these probes to specific membranes are emerging. In this …

Water-soluble red fluorescent protein dimers for hypoxic two-photon photodynamic therapy

W Feng, Y Qian - Journal of Materials Chemistry B, 2024‏ - pubs.rsc.org
In this study, two water-soluble red fluorescent protein (RFP) dimers, FP2R′ and FP2R′′,
were synthesized by linking two phenothiazine-based RFP chromophore analogues through …

Multiple Photoisomerization Pathways of the Green Fluorescent Protein Chromophore in a Reversibly Photoswitchable Fluorescent Protein: Insights from Quantum …

YH Zhu, XX Liu, Q Fang, XY Liu… - The Journal of Physical …, 2023‏ - ACS Publications
Herein, we have employed a combined CASPT2//CASSCF approach within the quantum
mechanics/molecular mechanics (QM/MM) framework to explore the early time …

Lights up on organelles: Optogenetic tools to control subcellular structure and organization

TC Kichuk, C Carrasco‐López… - WIREs Mechanisms of …, 2021‏ - Wiley Online Library
Since the neurobiological inception of optogenetics, light‐controlled molecular perturbations
have been applied in many scientific disciplines to both manipulate and observe cellular …