[HTML][HTML] Engineering enzyme access tunnels
Enzymes are efficient and specific catalysts for many essential reactions in biotechnological
and pharmaceutical industries. Many times, the natural enzymes do not display the catalytic …
and pharmaceutical industries. Many times, the natural enzymes do not display the catalytic …
[HTML][HTML] Tunnel engineering of gas-converting enzymes for inhibitor retardation and substrate acceleration
SM Kim, SH Kang, BW Jeon, YH Kim - Bioresource Technology, 2024 - Elsevier
Carbon monoxide dehydrogenase (CODH), formate dehydrogenase (FDH), hydrogenase
(H2ase), and nitrogenase (N2ase) are crucial enzymatic catalysts that facilitate the …
(H2ase), and nitrogenase (N2ase) are crucial enzymatic catalysts that facilitate the …
Expanding the Cation Cage: Squalene-Hopene Cyclase-Mediated Enantioselective Semipinacol Rearrangement
A Schneider, J Ruppert, TB Lystbæk, S Bastian… - ACS …, 2023 - ACS Publications
Squalene-hopene cyclases (SHCs) are the biocatalytic pendant to asymmetric Brønsted-
acid catalysis and thus comprise enormous synthetic potential. Nevertheless, their substrate …
acid catalysis and thus comprise enormous synthetic potential. Nevertheless, their substrate …
Cooperative Conformational Transitions Underpin the Activation Heat Capacity in the Temperature Dependence of Enzyme Catalysis
EJ Walker, CJ Hamill, R Crean, MS Connolly… - ACS …, 2024 - ACS Publications
Many enzymes display non-Arrhenius behavior with curved Arrhenius plots in the absence
of denaturation. There has been significant debate about the origin of this behavior and …
of denaturation. There has been significant debate about the origin of this behavior and …
Squalene hopene cyclases are protonases for stereoselective Brønsted acid catalysis
SC Hammer, A Marjanovic, JM Dominicus… - Nature chemical …, 2015 - nature.com
For many important reactions catalyzed in chemical laboratories, the corresponding
enzymes are missing, representing a restriction in biocatalysis. Although nature provides …
enzymes are missing, representing a restriction in biocatalysis. Although nature provides …
Key difference between transition state stabilization and ground state destabilization: increasing atomic charge densities before or during enzyme–substrate binding
D Chen, Y Li, X Li, X Hong, X Fan, T Savidge - Chemical Science, 2022 - pubs.rsc.org
The origin of the enormous catalytic power of enzymes has been extensively studied
through experimental and computational approaches. Although precise mechanisms are still …
through experimental and computational approaches. Although precise mechanisms are still …
Broadening the scope of biocatalysis engineering by tailoring enzyme microenvironment: a review
The rational design of catalysts that fine-tune/mimics the enzyme microenvironment remains
the subject of supreme interest. Several strategies moving from traditional to technologically …
the subject of supreme interest. Several strategies moving from traditional to technologically …
Alternative Active Site Confinement in Squalene–Hopene Cyclase Enforces Substrate Preorganization for Cyclization
Confinement of an enzyme's active site is critical to the efficiency of chemical reactions and
has been recognized as an important tool for catalysis to accelerate reactions by proximity …
has been recognized as an important tool for catalysis to accelerate reactions by proximity …
A pharmaceutical model for the molecular evolution of microbial natural products
Microbes are talented chemists with the ability to generate tremendously complex and
diverse natural products which harbor potent biological activities. Natural products are …
diverse natural products which harbor potent biological activities. Natural products are …
Squalene-hopene cyclases—evolution, dynamics and catalytic scope
Highlights•Squalene-hopene cyclases display a water-assisted entropy driven
mechanism.•The (α/α) 6 barrel fold of triterpene cyclases is evolutionary related to …
mechanism.•The (α/α) 6 barrel fold of triterpene cyclases is evolutionary related to …