Loop dynamics and the evolution of enzyme activity
In the early 2000s, Tawfik presented his 'New View'on enzyme evolution, highlighting the
role of conformational plasticity in expanding the functional diversity of limited repertoires of …
role of conformational plasticity in expanding the functional diversity of limited repertoires of …
Thermal stability enhancement: Fundamental concepts of protein engineering strategies to manipulate the flexible structure
Increasing the temperature by just a few degrees may lead to structural perturbation or
unfolding of the protein and consequent loss of function. The concepts of flexibility and …
unfolding of the protein and consequent loss of function. The concepts of flexibility and …
[HTML][HTML] Illuminating G-protein-coupling selectivity of GPCRs
Heterotrimetic G proteins consist of four subfamilies (G s, G i/o, G q/11, and G 12/13) that
mediate signaling via G-protein-coupled receptors (GPCRs), principally by receptors binding …
mediate signaling via G-protein-coupled receptors (GPCRs), principally by receptors binding …
Flexibility regulation of loops surrounding the tunnel entrance in cytochrome P450 enhanced substrate access substantially
In recent years, the engineering of flexible loops to improve enzyme properties has gained
attention in biocatalysis. Herein, we report a loop engineering strategy to improve the …
attention in biocatalysis. Herein, we report a loop engineering strategy to improve the …
An adaptable water-soluble molecular boat for selective separation of phenanthrene from isomeric anthracene
AB Sainaba, M Venkateswarulu… - Journal of the …, 2022 - ACS Publications
Anthracene crude oil is a common source of phenanthrene for its industrial use. The
isolation of phenanthrene from this source is a challenging task due to very similar physical …
isolation of phenanthrene from this source is a challenging task due to very similar physical …
Decrypting Allostery in Membrane-Bound K-Ras4B Using Complementary In Silico Approaches Based on Unbiased Molecular Dynamics Simulations
Protein functions are dynamically regulated by allostery, which enables conformational
communication even between faraway residues, and expresses itself in many forms, akin to …
communication even between faraway residues, and expresses itself in many forms, akin to …
Overcoming universal restrictions on metal selectivity by protein design
Selective metal coordination is central to the functions of metalloproteins:, each
metalloprotein must pair with its cognate metallocofactor to fulfil its biological role. However …
metalloprotein must pair with its cognate metallocofactor to fulfil its biological role. However …
Role of distal sites in enzyme engineering
J Gu, Y Xu, Y Nie - Biotechnology Advances, 2023 - Elsevier
The limitations associated with natural enzyme catalysis have triggered the rise of the field of
protein engineering. Traditional rational design was based on the analysis of protein …
protein engineering. Traditional rational design was based on the analysis of protein …
Benchmarking the accuracy of AlphaFold 2 in loop structure prediction
AO Stevens, Y He - Biomolecules, 2022 - mdpi.com
The inhibition of protein–protein interactions is a growing strategy in drug development. In
addition to structured regions, many protein loop regions are involved in protein–protein …
addition to structured regions, many protein loop regions are involved in protein–protein …
Enzyme fusions in biocatalysis: coupling reactions by pairing enzymes
FS Aalbers, MW Fraaije - ChemBioChem, 2019 - Wiley Online Library
One approach to bringing enzymes together for multienzyme biocatalysis is genetic fusion.
This enables the production of multifunctional enzymes that can be used for whole‐cell …
This enables the production of multifunctional enzymes that can be used for whole‐cell …