The historical reliance of biological research on the use of animal models has sometimes
made it challenging to address questions that are specific to the understanding of human …

Organoids are in vitro miniaturized and simplified model systems of organs that have gained
enormous interest for modelling tissue development and disease, and for personalized …

The WNT signal transduction cascade is a main regulator of development throughout the
animal kingdom. Wnts are also key drivers of most types of tissue stem cells in adult …

In recent years, stem cell therapy has become a very promising and advanced scientific
research topic. The development of treatment methods has evoked great expectations. This …

Recent advances in 3D culture technology allow embryonic and adult mammalian stem cells
to exhibit their remarkable self-organizing properties, and the resulting organoids reflect key …

In vitro cancer cultures, including three-dimensional organoids, typically contain exclusively
neoplastic epithelium but require artificial reconstitution to recapitulate the tumor …

Matrigel, a basement-membrane matrix extracted from Engelbreth–Holm–Swarm mouse
sarcomas, has been used for more than four decades for a myriad of cell-culture …

Adult stem cell–based organoid technology is a versatile tool for the generation and long-
term maintenance of near-native 3D epithelial tissues in vitro. The generation of cancer …

Organoids are three-dimensional (3D) miniature structures cultured in vitro produced from
either human pluripotent stem cells (hPSCs) or adult stem cells (AdSCs) derived from …

Tissue and organ biology are very challenging to study in mammals, and progress can be
hindered, particularly in humans, by sample accessibility and ethical concerns. However …