Advances and challenges in biotechnological production of chondroitin sulfate and its oligosaccharides

W Zhang, R Xu, J Chen, H **ong, Y Wang… - International journal of …, 2023‏ - Elsevier
Chondroitin sulfate (CS) is a member of glycosaminoglycans (GAGs) and has critical
physiological functions. CS is widely applied in medical and clinical fields. Currently, the …

Advances in green bioproduction of marine and glycosaminoglycan oligosaccharides

C Deng, M Zhao, Q Zhao, L Zhao - Carbohydrate Polymers, 2023‏ - Elsevier
Functional oligosaccharides are a class of low-polymerization sugars with physiological
functions, showing great potential in the fields of food, medicine, and agriculture, with …

Biosynthesis of non-animal chondroitin sulfate from methanol using genetically engineered Pichia pastoris

X **, W Zhang, Y Wang, J Sheng, R Xu, J Li, G Du… - Green …, 2021‏ - pubs.rsc.org
Chondroitin sulfate (CS) is widely applied in the medical, clinical, and nutraceutical fields.
However, all commercialized CS is extracted from animal tissues, which is associated with …

Enhancing the expression of chondroitin 4-O-sulfotransferase for one-pot enzymatic synthesis of chondroitin sulfate A

W Zhang, P Zhang, H Wang, R Xu, Z **e, Y Wang… - Carbohydrate …, 2024‏ - Elsevier
Chondroitin sulfate (CS) stands as a pivotal compound in dietary supplements for
osteoarthritis treatment, propelling significant interest in the biotechnological pursuit of …

Marine-derived uronic acid-containing polysaccharides: Structures, sources, production, and nutritional functions

M Yang, D Zhou, H **ao, X Fu, Q Kong, C Zhu… - Trends in Food Science …, 2022‏ - Elsevier
Background Uronic acid-containing polysaccharides (UACPs) are a class of
polysaccharides containing at least one type of uronic acid (UA) and have been widely used …

Biosynthesis of non-sulfated high-molecular-weight glycosaminoglycans and specific-sized oligosaccharides

L Hu, Y Wang, Y Hu, J Yin, L Wang, G Du, J Chen… - Carbohydrate …, 2022‏ - Elsevier
Non-sulfated forms of glycosaminoglycans (NSGAGs) including hyaluronan, chondroitin and
heparosan with high-molecular-weight (HMW) are extensively used biomaterials, while …

Microbial synthesis of glycosaminoglycans and their oligosaccharides

K Liu, L Guo, X Chen, L Liu, C Gao - Trends in Microbiology, 2023‏ - cell.com
Compared with chemical synthesis and tissue extraction methods, microbial synthesis of
glycosaminoglycans (GAGs) is attractive because of the advantages of eco-friendly …

Microbial cell surface engineering for high-level synthesis of bio-products

X Wu, J Liu, Z Liu, G Gong, J Zha - Biotechnology advances, 2022‏ - Elsevier
Microbial cell surface layers, which mainly include the cell membrane, cell wall, periplasmic
space, outer membrane, capsules, S-layers, pili, and flagella, control material exchange …

Enzymatic production of chondroitin oligosaccharides and its sulfate derivatives

W Zhang, R Xu, X **, Y Wang, L Hu… - … in Bioengineering and …, 2022‏ - frontiersin.org
Chondroitin sulfate (CS) has a wide range of physiological functions and clinical
applications. However, the biosynthesis of chondroitin oligosaccharides (o-CHs) and sulfate …

Optimizing the sulfation-modification system for scale preparation of chondroitin sulfate A

X **, Q Li, Y Wang, W Zhang, R Xu, J Li, G Du… - Carbohydrate …, 2020‏ - Elsevier
Chondroitin sulfate (CS) extracted from animal tissues has been widely used as
nutraceutical and pharmaceutical products for osteoarthritis treatment. Here we developed …