Disrupted protein folding or decreased protein stability can lead to the accumulation of
(partially) un‐or misfolded proteins, which ultimately cause the formation of protein …

Monoclonal antibodies (mAbs) are a fast-growing class of biopharmaceuticals. They are
widely used in the identification and detection of cell makers, serum analytes, and …

Gene therapy is a promising therapeutic approach for genetic and acquired diseases
nowadays. Among DNA delivery vectors, recombinant adeno‐associated virus (rAAV) is one …

Analytical size-exclusion chromatography (SEC), commonly used for the determination of
the molecular weight of proteins and protein-protein complexes in solution, is a relative …

Mass spectrometry (MS) is increasingly being used to probe the structure and dynamics of
proteins and the complexes they form with other macromolecules. There are now several …

Understanding the structure, functionalities and biology of functional amyloids is an issue of
emerging interest. Inclusion bodies, namely protein clusters formed in recombinant bacteria …

Viral vectors are an emerging, exciting class of biologics whose application in vaccines,
oncology, and gene therapy has grown exponentially in recent years. Following first …

The transition to continuous biomanufacturing is considered the next step to reduce costs
and improve process robustness in the biopharmaceutical industry, while also improving …

HPLC is established as a fast convenient analytical technology for characterizing the content
of empty and full capsids in purified samples containing adeno-associated virus (AAV). UV …

Ion-exchange chromatography coupled to light scattering detectors represents a fast and
simple analytical method for the assessment of multiple critical quality attributes (CQA) in …