Genome-wide analysis of genes involved in efflux function and regulation within Escherichia coli and Salmonella enterica serovar Typhimurium

ER Holden, M Yasir, AK Turner, J Wain… - …, 2023 - microbiologyresearch.org
The incidence of multidrug-resistant bacteria is increasing globally, with efflux pumps being
a fundamental platform limiting drug access and synergizing with other mechanisms of …

Collateral sensitivity increases the efficacy of a rationally designed bacteriophage combination to control Salmonella enterica

L Acton, HV Pye, G Thilliez, R Kolenda… - Journal of …, 2024 - journals.asm.org
The ability of virulent bacteriophages to lyse bacteria influences bacterial evolution, fitness,
and population structure. Knowledge of both host susceptibility and resistance factors is …

[HTML][HTML] Common food preservatives impose distinct selective pressures on Salmonella Typhimurium planktonic and biofilm populations

J Abi Assaf, ER Holden, E Trampari, MA Webber - Food Microbiology, 2024 - Elsevier
Food preservatives are crucial in controlling microbial growth in processed foods to maintain
food safety. Bacterial biofilms pose a threat in the food chain by facilitating persistence on a …

Identification of pathways required for Salmonella to colonize alfalfa using TraDIS-Xpress

ER Holden, J Abi Assaf, H Al-Khanaq… - Applied and …, 2024 - journals.asm.org
Enteropathogenic bacteria, such as Salmonella, have been linked to numerous fresh
produce outbreaks, posing a significant public health threat. The ability of Salmonella to …

Comparison of the genetic basis of biofilm formation between Salmonella Typhimurium and Escherichia coli

ER Holden, M Yasir, AK Turner… - Microbial …, 2022 - microbiologyresearch.org
Most bacteria can form biofilms, which typically have a life cycle from cells initially attaching
to a surface before aggregation and growth produces biomass and an extracellular matrix …

Mechanisms of action and synergies of a novel lipid IVA biosynthesis inhibitor

ER Holden, M Yasir, AK Turner, MA Webber, I Charles… - bioRxiv, 2023 - biorxiv.org
The development of novel antimicrobials provides additional treatment options for infectious
diseases, including antimicrobial resistant infections. There are many hurdles to …

Golden Gate-Assisted Gene Doctoring for Streamlined and Efficient Recombineering in Bacteria

NM Thomson - Golden Gate Cloning: Methods and Protocols, 2024 - Springer
Gene Doctoring is a genetic modification technique for E. coli and related bacteria, in which
the Red-recombinase from bacteriophage λ mediates chromosomal integration of a …

Investigating how Salmonella colonise alfalfa using a whole genome screen

ER Holden, H Al-Khanaq, N Vimont, MA Webber… - bioRxiv, 2023 - biorxiv.org
Enteropathogenic bacteria including Salmonella regularly cause outbreaks of infection from
fresh produce posing a significant public health threat. Salmonella's ability to persist on fresh …

Massively parallel transposon mutagenesis to identify relationships between biofilm formation and efflux activity in Enterobacteriaceae

E Holden - 2021 - ueaeprints.uea.ac.uk
Bacteria are usually found as part of structured, aggregated communities called biofilms.
Progression through the biofilm life cycle requires temporally controlled gene expression to …