Single-molecule super-resolution fluorescence microscopy and single-particle tracking are
two imaging modalities that illuminate the properties of cells and materials on spatial scales …

The initial steps toward optical detection and spectroscopy of single molecules in
condensed matter arose out of the study of inhomogeneously broadened optical absorption …

The point spread function (PSF) of a microscope describes the image of a point emitter.
Knowing the accurate PSF model is essential for various imaging tasks, including single …

To extract from an image of a single nanoscale object maximum physical information about
its position, we propose and demonstrate a framework for pupil-plane modulation for 3D …

Imaging of both the positions and orientations of single fluorophores, termed single-
molecule orientation-localization microscopy, is a powerful tool for the study of biochemical …

We employ a novel framework for information-optimal microscopy to design a family of point
spread functions (PSFs), the Tetrapod PSFs, which enable high-precision localization of …

Optical contrasts in microscopy are sensitive to light polarization, whose interaction with
molecular dipoles provides an important lever for probing molecular orientation. Polarization …

Synthetic peptides that self-assemble into cross-β fibrils are versatile building blocks for
engineered biomaterials due to their modularity and biocompatibility, but their structural and …

Fundamental properties of light unavoidably impose features on images collected using
fluorescence microscopes. Accounting for these features is often critical in quantitatively …

Essential cellular functions as diverse as genome maintenance and tissue morphogenesis
rely on the dynamic organization of filamentous assemblies. For example, the precise …