Biological nanopore approach for single‐molecule protein sequencing

ZL Hu, MZ Huo, YL Ying, YT Long - Angewandte Chemie, 2021 - Wiley Online Library
Proteins are responsible for the occurrence and treatment of many diseases, and therefore
protein sequencing will revolutionize proteomics and clinical diagnostics. Biological …

The application of single molecule nanopore sensing for quantitative analysis

Y Wu, JJ Gooding - Chemical Society Reviews, 2022 - pubs.rsc.org
Nanopore-based sensors typically work by monitoring transient pulses in conductance via
current–time traces as molecules translocate through the nanopore. The unique property of …

Peptide sequencing based on host–guest interaction-assisted nanopore sensing

Y Zhang, Y Yi, Z Li, K Zhou, L Liu, HC Wu - Nature methods, 2024 - nature.com
Direct protein sequencing technologies with improved sensitivity and throughput are still
needed. Here, we propose an alternative method for peptide sequencing based on …

Nanopore-based protein identification

M Afshar Bakshloo, JJ Kasianowicz… - Journal of the …, 2022 - ACS Publications
The implementation of a reliable, rapid, inexpensive, and simple method for whole-proteome
identification would greatly benefit cell biology research and clinical medicine. Proteins are …

Single Molecule Ratcheting Motion of Peptides in a Mycobacterium smegmatis Porin A (MspA) Nanopore

S Yan, J Zhang, Y Wang, W Guo, S Zhang, Y Liu… - Nano …, 2021 - ACS Publications
Diverse functions of proteins, including synthesis, catalysis, and signaling, result from their
highly variable amino acid sequences. The technology allowing for direct analysis of protein …

FraC nanopores with adjustable diameter identify the mass of opposite-charge peptides with 44 dalton resolution

G Huang, A Voet, G Maglia - Nature communications, 2019 - nature.com
A high throughput single-molecule method for identifying peptides and sequencing proteins
based on nanopores could reduce costs and increase speeds of sequencing, allow the …

Nanopore detection of metal ions: Current status and future directions

GM Roozbahani, X Chen, Y Zhang, L Wang… - Small …, 2020 - Wiley Online Library
In this review, recent research efforts that aimed at develo** nanopore sensors for
detection of metal ions, which play a crucial role in environmental safety and human health …

Biological nanopores: Engineering on demand

A Crnković, M Srnko, G Anderluh - Life, 2021 - mdpi.com
Nanopore-based sensing is a powerful technique for the detection of diverse organic and
inorganic molecules, long-read sequencing of nucleic acids, and single-molecule analyses …

Enhanced identification of Tau acetylation and phosphorylation with an engineered aerolysin nanopore

MZ Huo, ZL Hu, YL Ying, YT Long - Proteomics, 2022 - Wiley Online Library
Posttranslational modifications (PTMs) affect protein function/dysfunction, playing important
roles in the occurrence and development of tauopathies including Alzheimer's disease. PTM …

Detection of peptides with different charges and lengths by using the aerolysin nanopore

S Li, C Cao, J Yang, YT Long - ChemElectroChem, 2019 - Wiley Online Library
The aerolysin nanopore has a high sensitivity for molecular sensing, owing to its super‐
narrow diameter and highly charged lumen structure. Here, taking advantages of its high …