Fundamental properties of light unavoidably impose features on images collected using
fluorescence microscopes. Accounting for these features is often critical in quantitatively …

Single-molecule localization microscopy in a typical wide-field setup has been widely used
for investigating subcellular structures with super resolution; however, field-dependent …

An outstanding challenge in single-molecule localization microscopy is the accurate and
precise localization of individual point emitters in three dimensions in densely labeled …

The point spread function (PSF) of a microscope describes the image of a point emitter.
Knowing the accurate PSF model is essential for various imaging tasks, including single …

High-throughput microscopy is vital for screening applications, where three-dimensional
(3D) cellular models play a key role. However, due to defocus susceptibility, current 3D high …

Interactions between biomolecules are characterized by where they occur and how they are
organized, eg, the alignment of lipid molecules to form a membrane. However, spatial and …

Estimation of optical aberrations from volumetric intensity images is a key step in sensorless
adaptive optics for 3D microscopy. Recent approaches based on deep learning promise …

Volumetric super-resolution microscopy typically encodes the 3D position of single-molecule
fluorescence into a 2D image by changing the shape of the point spread function (PSF) as a …

Accurate characterization of the microscopic point spread function (PSF) is crucial for
achieving high-performance localization microscopy (LM). Traditionally, LM assumes a …

Diffractive optical elements (DOEs) are used to shape the wavefront of incident light. This
can be used to generate practically any pattern of interest, albeit with varying efficiency. A …