Getting to know the neighborhood: using proximity-dependent biotinylation to characterize protein complexes and map organelles
The use of proximity-dependent biotinylation approaches combined with mass spectrometry
(eg BioID and APEX) has revolutionized the study of protein–protein interactions and …
(eg BioID and APEX) has revolutionized the study of protein–protein interactions and …
Subcellular proteomics
The eukaryotic cell is compartmentalized into subcellular niches, including membrane-
bound and membrane-less organelles. Proteins localize to these niches to fulfil their …
bound and membrane-less organelles. Proteins localize to these niches to fulfil their …
A proximity-dependent biotinylation map of a human cell
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct
biochemical processes into discrete subcellular locations. Microscopy and biochemical …
biochemical processes into discrete subcellular locations. Microscopy and biochemical …
[HTML][HTML] High-density proximity map** reveals the subcellular organization of mRNA-associated granules and bodies
Summary mRNA processing, transport, translation, and ultimately degradation involve a
series of dedicated protein complexes that often assemble into large membraneless …
series of dedicated protein complexes that often assemble into large membraneless …
Map** of the plant SnRK1 kinase signalling network reveals a key regulatory role for the class II T6P synthase-like proteins
The central metabolic regulator SnRK1 controls plant growth and survival upon activation by
energy depletion, but detailed molecular insight into its regulation and downstream targets is …
energy depletion, but detailed molecular insight into its regulation and downstream targets is …
SARS‐CoV‐2–host proteome interactions for antiviral drug discovery
Abstract Treatment options for COVID‐19, caused by SARS‐CoV‐2, remain limited.
Understanding viral pathogenesis at the molecular level is critical to develop effective …
Understanding viral pathogenesis at the molecular level is critical to develop effective …
A high-density human mitochondrial proximity interaction network
H Antonicka, ZY Lin, A Janer, MJ Aaltonen… - Cell metabolism, 2020 - cell.com
We used BioID, a proximity-dependent biotinylation assay with 100 mitochondrial baits from
all mitochondrial sub-compartments, to create a high-resolution human mitochondrial …
all mitochondrial sub-compartments, to create a high-resolution human mitochondrial …
Map** the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins
(RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to …
(RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to …
Identification and functional characterization of transcriptional activators in human cells
Transcription is orchestrated by thousands of transcription factors (TFs) and chromatin-
associated proteins, but how these are causally connected to transcriptional activation is …
associated proteins, but how these are causally connected to transcriptional activation is …
Human transcription factor protein interaction networks
H Göös, M Kinnunen, K Salokas, Z Tan, X Liu… - Nature …, 2022 - nature.com
Transcription factors (TFs) interact with several other proteins in the process of
transcriptional regulation. Here, we identify 6703 and 1536 protein–protein interactions for …
transcriptional regulation. Here, we identify 6703 and 1536 protein–protein interactions for …