DNA synthesis and assembly primarily revolve around the innovation and refinement of
tools that facilitate the creation of specific genes and the manipulation of entire genomes …

DNA assembly is one of the most important foundational technologies for synthetic biology
and metabolic engineering. Since the development of the restriction digestion and ligation …

One-step multiple gene disruption in the model organism Saccharomyces cerevisiae is a
highly useful tool for both basic and applied research, but it remains a challenge. Here, we …

Large-scale data acquisition and analysis are often required in the successful
implementation of the design, build, test, and learn (DBTL) cycle in biosystems design …

Abstract CRISPR/Cas9 or Cpf1-introduced double strand break dramatically decreases
bacterial cell survival rate, which hampers multiplex genome editing in bacteria. In addition …

Genome editing facilitated by Cas9‐based RNA‐guided nucleases (RGN s) is becoming an
increasingly important and popular technique for reverse genetics in both model and non …

Genome editing is the practice of making predetermined and precise changes to a genome
by controlling the location of DNA DSBs (double-strand breaks) and manipulating the cell's …

Background Metabolic engineering has expanded from a focus on designs requiring a small
number of genetic modifications to increasingly complex designs driven by advances in …

Performing saturation editing of chromosomal genes will enable the study of genetic variants
in situ and facilitate protein and cell engineering. However, current in vivo editing of …

Recent advances in the targeted modification of complex eukaryotic genomes have
unlocked a new era of genome engineering. From the pioneering work using zinc-finger …