Metabolic engineering aims to improve the production of economically valuable molecules
through the genetic manipulation of microbial metabolism. While the discipline is a little over …

E. coli is most preferred system used for the production of recombinant proteins in bacteria
and the availability of improved genetic tools/methods are making it more valuable than …

We have constructed a series of plasmid vectors (pBAD vectors) containing the PBAD
promoter of the araBAD (arabinose) operon and the gene encoding the positive and …

Based on parameters governing promoter activity and using regulatory elements of the lac,
ara and tet operon transcription control sequences were composed which permit the …

During the proteomics period, the growth in the use of recombinant proteins has increased
greatly in the recent years. Bacterial systems remain most attractive due to low cost, high …

Progress in our understanding of several biological processes promises to broaden the
usefulness of Escherichia coli as a tool for gene expression. There is an expanding choice …

Glucose oxidase is a subset of oxidoreductase enzymes that catalyzes the transfer of
electrons from an oxidant to a reductant. Glucose oxidases use oxygen as an external …

Highlights•The need for recombinant proteins both as biopharmaceuticals and for structural
studies in drug development is outlined.•The basis of the production of recombinant proteins …

Synthetic control of gene expression is critical for metabolic engineering efforts. Specifically,
precise control of key pathway enzymes (heterologous or native) can help maximize product …

Recombinant protein expression in Escherichia coli (E. coli) is simple, fast, inexpensive, and
robust, with the expressed protein comprising up to 50 percent of the total cellular protein …