Trends in develo** one-pot CRISPR diagnostics strategies
L Chen, M Hu, X Zhou - Trends in Biotechnology, 2024 - cell.com
The integration of nucleic acid amplification (NAA) with the CRISPR detection system has
led to significant advancements and opportunities for development in molecular diagnostics …
led to significant advancements and opportunities for development in molecular diagnostics …
One-pot diagnostic methods based on CRISPR/Cas and Argonaute nucleases: strategies and perspectives
X Ye, H Wu, J Liu, J **ang, Y Feng, Q Liu - Trends in Biotechnology, 2024 - cell.com
Abstract CRISPR/Cas and Argonaute (Ago) proteins, which target specific nucleic acid
sequences, can be applied as diagnostic tools. Despite high specificity and efficiency …
sequences, can be applied as diagnostic tools. Despite high specificity and efficiency …
Light‐Start CRISPR‐Cas12a Reaction with Caged crRNA Enables Rapid and Sensitive Nucleic Acid Detection
M Hu, R Liu, Z Qiu, F Cao, T Tian, Y Lu… - Angewandte …, 2023 - Wiley Online Library
The clustered regularly interspaced short palindromic repeats (CRISPR) system is a
promising platform for nucleic acid detection. Regulating the CRISPR reaction would be …
promising platform for nucleic acid detection. Regulating the CRISPR reaction would be …
Glow-in-the-dark infectious disease diagnostics using Crispr-Cas9-based Split luciferase complementation
HJ van der Veer, EA van Aalen… - ACS Central …, 2023 - ACS Publications
Nucleic acid detection methods based on CRISPR and isothermal amplification techniques
show great potential for point-of-care diagnostic applications. However, most current …
show great potential for point-of-care diagnostic applications. However, most current …
Ultrasensitive detection of mirna via Crispr/Cas12a coupled with Strand displacement amplification reaction
MicroRNA (miRNA) is a promising biomarker for the diagnosis, monitoring, and prognostic
evaluation of diseases, especially cancer. The existing miRNA detection methods usually …
evaluation of diseases, especially cancer. The existing miRNA detection methods usually …
Regulating the trans-Cleavage Activity of CRISPR/Cas12a by Using an Elongation-Caged Single-Stranded DNA Activator and the Biosensing Applications
X Fei, C Lei, W Ren, X Liu, C Liu - Analytical Chemistry, 2023 - ACS Publications
The CRISPR/Cas12a system exhibits extraordinary capability in the field of biosensing and
molecular diagnosis due to its trans-cleavage ability. However, it is still desirable for precise …
molecular diagnosis due to its trans-cleavage ability. However, it is still desirable for precise …
An aM-level sensitive cascade CRISPR-Dx system (ASCas) for rapid detection of RNA without pre-amplification
Y Zhang, Y Chen, Q Zhang, Y Liu, X Zhang - Biosensors and Bioelectronics, 2023 - Elsevier
The CRISPR/Cas system is known as one of the directions of the next generation of
mainstream molecular diagnostic technology. However, most current CRISPR/Cas …
mainstream molecular diagnostic technology. However, most current CRISPR/Cas …
[HTML][HTML] A universal all-in-one RPA-Cas12a strategy with de novo autodesigner and its application in on-site ultrasensitive detection of DNA and RNA viruses
C Lin, F Chen, D Huang, W Li, C He, Y Tang… - Biosensors and …, 2023 - Elsevier
Revolutionary all-in-one RPA-CRISPR assays are rapidly becoming the most sought-after
tools for point-of-care testing (POCT) due to their high sensitivity and ease of use. Despite …
tools for point-of-care testing (POCT) due to their high sensitivity and ease of use. Despite …
A one-pot RPA-CRISPR detection method for point-of-care testing of Enterocytozoon hepatopenaei infection in shrimp
P Wang, X Zhang, H Shen, Q Lu, B Li, X Liu… - Sensors and Actuators B …, 2023 - Elsevier
The pathogen Enterocytozoon hepatopenaei (EHP) has significantly affected the shrimp
farming industry since the last decade. For effective prevention, accurate and reliable …
farming industry since the last decade. For effective prevention, accurate and reliable …
One-step reverse-transcription recombinase-aided amplification CRISPR/Cas12a-based lateral flow assay for fast field screening and accurate differentiation of four …
Z Zhao, S Wang, Z Dong, Q Fan, R Lei… - Journal of Agricultural …, 2023 - ACS Publications
Several tobamoviruses cause substantial economic losses to tomato and pepper crops
globally, especially the pepper mild mosaic virus (PMMoV), tomato brown rugose fruit virus …
globally, especially the pepper mild mosaic virus (PMMoV), tomato brown rugose fruit virus …