Genomes have complex three-dimensional architectures. The recent convergence of
genetic, biochemical, biophysical, and cell biological methods has uncovered several …

The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered
regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a …

The association of nuclear DNA with histones to form chromatin is essential for temporal and
spatial control of eukaryotic genomes. In this study, we examined the physical state of …

In mammals, HP1-mediated heterochromatin forms positionally and mechanically stable
genomic domains even though the component HP1 paralogs, HP1α, HP1β, and HP1γ …

The long-dreamed-of capability of monitoring the molecular machinery in living systems has
not been realized yet, mainly due to the technical limitations of current sensing technologies …

Enhancers are important regulatory elements that can control gene activity across vast
genetic distances. However, the underlying nature of this regulation remains obscured …

Chromatin is organized into higher-order structures that form subcompartments in
interphase nuclei. Different categories of specialized enzymes act on chromatin and …

To achieve guide RNA (gRNA) multiplexing and an efficient delivery of tens of distinct
gRNAs into single cells, we developed a molecular assembly strategy termed chimeric array …

The primary regulators of metazoan gene expression are enhancers, originally functionally
defined as DNA sequences that can activate transcription at promoters in an orientation …

Several general principles of global 3D genome organization have recently been
established, including non-random positioning of chromosomes and genes in the cell …