Label-free microfluidic cell sorting and detection for rapid blood analysis

N Lu, HM Tay, C Petchakup, L He, L Gong, KK Maw… - Lab on a Chip, 2023 - pubs.rsc.org
Blood tests are considered as standard clinical procedures to screen for markers of diseases
and health conditions. However, the complex cellular background (> 99.9% RBCs) and …

Physical phenotype of blood cells is altered in COVID-19

M Kubánková, B Hohberger, J Hoffmanns, J Fürst… - Biophysical journal, 2021 - cell.com
Clinical syndrome coronavirus disease 2019 (COVID-19) induced by severe acute
respiratory syndrome coronavirus 2 is characterized by rapid spreading and high mortality …

Recent advances in deformation-assisted microfluidic cell sorting technologies

J Sun, X Huang, J Chen, R **ang, X Ke, S Lin, W Xuan… - Analyst, 2023 - pubs.rsc.org
Cell sorting is an essential prerequisite for cell research and has great value in life science
and clinical studies. Among the many microfluidic cell sorting technologies, label-free …

High-throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry

B Fregin, F Czerwinski, D Biedenweg, S Girardo… - Nature …, 2019 - nature.com
In life sciences, the material properties of suspended cells have attained significance close
to that of fluorescent markers but with the advantage of label-free and unbiased sample …

Deformability-induced lift force in spiral microchannels for cell separation

E Guzniczak, O Otto, G Whyte, N Willoughby… - Lab on a Chip, 2020 - pubs.rsc.org
Cell sorting and isolation from a heterogeneous mixture is a crucial task in many aspects of
cell biology, biotechnology and medicine. Recently, there has been an interest in methods …

Effects of vimentin on the migration, search efficiency, and mechanical resilience of dendritic cells

MR Shaebani, L Stankevicins, D Vesperini… - Biophysical …, 2022 - cell.com
Dendritic cells use amoeboid migration to pass through narrow passages in the extracellular
matrix and confined tissue in search for pathogens and to reach the lymph nodes and alert …

High-throughput adjustable deformability cytometry utilizing elasto-inertial focusing and virtual fluidic channel

Z Zhou, C Ni, Z Zhu, Y Chen, Z Ni, N **ang - Lab on a Chip, 2023 - pubs.rsc.org
Cell mechanical properties provide a label-free marker for indicating cell states and disease
processes. Although microfluidic deformability cytometry has demonstrated great potential …

High-throughput cell and spheroid mechanics in virtual fluidic channels

MH Panhwar, F Czerwinski, VAS Dabbiru… - Nature …, 2020 - nature.com
Microfluidics by soft lithography has proven to be of key importance for biophysics and life
science research. While being based on replicating structures of a master mold using …

Real-time deformability cytometry reveals sequential contraction and expansion during neutrophil priming

KR Bashant, A Vassallo, C Herold… - Journal of leukocyte …, 2019 - academic.oup.com
It has become increasingly apparent that the biomechanical properties of neutrophils impact
on their trafficking through the circulation and in particularly through the pulmonary capillary …

Cytoskeletal vimentin regulates cell size and autophagy through mTORC1 signaling

P Mohanasundaram, LS Coelho-Rato, MK Modi… - PLoS …, 2022 - journals.plos.org
The nutrient-activated mTORC1 (mechanistic target of rapamycin kinase complex 1)
signaling pathway determines cell size by controlling mRNA translation, ribosome …