Correct expression of the genetic code at translation is directly correlated with tRNA identity.
This survey describes the molecular signals in tRNAs that trigger specific aminoacylations …

▪ Abstract Positive strand RNA viral genomes are unique in the viral world in serving a dual
role as mRNA and replicon. Since the origin of the minus-strand RNA replication …

A modified polymerase chain reaction (PCR) was developed to introduce random point
mutations into cloned genes. The modifications were made to decrease the fidelity of Taq …

Olig1 and Olig2 are closely related basic helix-loop-helix (bHLH) transcription factors that
are expressed in myelinating oligodendrocytes and their progenitor cells in the develo** …

A recombinant plasmid was constructed with six synthetic DNA oligomers such that the DNA
sequence corresponding to yeast tRNA (Phe) is flanked by a T7 promoter and a BstNI …

We constructed full-length cDNA clones of Sindbis virus that can be transcribed in vitro by
SP6 RNA polymerase to produce infectious genome-length transcripts. Viruses produced …

The in vitro synthesis of extraneous RNA sequences by SP6 and T7 RNA polymerases from
specific DNA templates is described. Transcription of templates prepared by digestion with …

Direct sequencing of human mitochondrial tRNALys shows the absence of editing and the
occurrence of six modified nucleotides (m1A9, m2G10, Ψ27, Ψ28 and hypermodified …

Abstract The genomes of many (+)-stranded RNA viruses, including plant viruses and
alphaviruses, consist of polycistronic RNAs whose internal genes are expressed via …

Transfer RNA-like structures (TLSs) that are sophisticated functional mimics of tRNAs are
found at the 3′-termini of the genomes of a number of plant positive strand RNA viruses …